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SpyRho 555 is a fluorogenic dye ligand for the RNA aptamer RhoBAST developed by the lab of Murat Sunbul in Heidelberg University. SpyRho 555 is a rhodamine dye that is cell permeable, highly fluorogenic and photostable. With its high affinity and fast on/off binding kinetics to its cognate aptamer RhoBAST, it allows overcoming the main issues of live cell RNA imaging. SpyRho 555 is highly suited for widefield, confocal, STED or single molecule localization microscopy in a PAINT like setup. SpyRho 555 enables multicolor imaging with SPY505, SPY595, SPY650, SPY700, SiR and GFP. SpyRho 555 can be imaged with standard TMR or Cy3 settings