Labelling

This kit is an all-in-one kit that allows visualization of the endocytosis uptake of a target substance. The NH2-Reactive AcidSensor (fluorescent probe) included in the kit has an intramolecular active ester group that forms a stable covalent bond when mixed with an amino group-containing target substance (protein). The AcidSensor label can be excited at 633 nm, allowing for multiple staining with green or red fluorescence.

This kit includes lysosome staining dyes, pHLys Red (pH dependent), and LysoPrime Green (pH-independent). The pHLys Red and LysoPrime Green accumulate in the intact lysosomes. The fluorescence intensity of pHLys Red is enhanced as the acidity increases, and weak fluorescence is observed when lysosomes are neutralized due to the lysosomal dysfunction. On the other hand, LysoPrime Green gives stable emissions even lysosomes are neutralized. Lysosomal pH and lysosomal mass can be measured by combining these two dyes.

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・Accurate pH changes can be detected by measuring lysosomal pH and volume changes in the same sample ・All-in-one Kit with Lysosome Acidification Inhibitor included ・Compatible with imaging and quantitative assay

Bright-Dtech 545-Tb (Green) without coupling

Bright-Dtech 545-Tb (Green) coupled to Biotin