Labelling

Water-soluble, substrate for sortase mediated labeling of proteins. Sortase catalyzes a transpeptidase reaction between a specific internal sequence of a protein and an amine group present on the N-terminus of triglycine recently has become an area of great interest. This method of labeling proteins has been denoted as “Sortagging”. BP Fluor 647 Gly-Gly-Gly is a water-soluble, pH-insensitive from pH 4 to pH 10, far-red-fluorescent probe. Its excitation ideally suited for the 633 nm or 647 nm laser lines. BP Fluor 647 is structurally similar to Alexa Fluor® 647, and spectrally is almost identical to Cy5 Dye, Alexa Fluor® 647, CF® 647 Dye, or any other Cyanine5 based fluorescent dyes.

Water-soluble, substrate for sortase mediated labeling of proteins. Sortase catalyzes a transpeptidase reaction between a specific internal sequence of a protein and an amine group present on the N-terminus of triglycine recently has become an area of great interest. This method of labeling proteins has been denoted as “Sortagging”. BP Fluor 680 is a bright and photostable near-IR dye that is spectrally identical to Alexa Fluor® 680, IRDye® 680RD and Cy5.5. BP Fluor 680 is often used for small animal imaging applications in the 700 nm wavelength and provides excellent clearance profiles in animals. Flow cytometry is another common application for this dye. BP Fluor 680 ideally suited for the 633 nm laser line.

Water-soluble, substrate for sortase mediated labeling of proteins. Sortase catalyzes a transpeptidase reaction between a specific internal sequence of a protein and an amine group present on the N-terminus of triglycine recently has become an area of great interest. This method of labeling proteins has been denoted as “Sortagging”. Cy7 is a bright and photostable near-IR probe that spectrally similar to Alexa Fluor® 750, DyLight® 750, and IRDye® 750 dye. The Cy7 DBCO is water-soluble, hydrophilic dye often a reagent of choice for assay where minimal non-specific binding and exceptional brightness is required. The fluorescence of Cy7 DBCO is pH insensitive from pH 4 to pH 10 and produces minimal autofluorescence of biological specimens in this region of the spectrum. Fluorescence of this long-wavelength Cyanine dye is not visible to the human eye but is readily detected by most imaging systems.

Water-soluble, substrate for sortase mediated labeling of proteins. Sortase catalyzes a transpeptidase reaction between a specific internal sequence of a protein and an amine group present on the N-terminus of triglycine recently has become an area of great interest. This method of labeling proteins has been denoted as “Sortagging”. BP Fluor 405 is a water-soluble, pH-insensitive from pH 4 to pH 10, blue-fluorescent probe with excitation ideally suited for the 407 nm spectral line of the krypton laser or the 408 nm violet laser diode. BP Fluor 405 is often used in multicolor applications, including flow cytometry and super-resolution microscopy using STORM.

Water-soluble, substrate for sortase mediated labeling of proteins. Sortase catalyzes a transpeptidase reaction between a specific internal sequence of a protein and an amine group present on the N-terminus of triglycine recently has become an area of great interest. This method of labeling proteins has been denoted as “Sortagging”. Cy3 is a water-soluble, red fluorescent pH-insensitive from pH 4 to pH 10 probe. Its excitation peak is ideally suited for the 532 nm or 555 nm laser lines and its absorption and emission spectra are almost identical to those of Alexa Fluor® 555, CF® 555 Dye or any other Cyanine3 based fluorescent dyes.

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HO-PEG-Fluor-647 TEA salts, MW 2,000 is a PEG-dye conjugate featuring a free alcohol and a Fluor 647 dye. Alcohol groups are versatile building blocks towards higher structural complexity while Fluor 647 is a bright far-red fluorophore with excitation and emission maxima of 650 nm and 671 nm respectively.

TFA Amine-PEG-Fluor-647 TEA salts, MW 2,000 is a PEG-dye conjugate featuring a primary amine and a Fluor 647 dye. Primary amines are versatile building blocks which are most easily employed in coupling reactions with carboxylic acids with the help of activators such as HATU or EDC. Fluor 647 is a bright far-red fluorophore with excitation and emission maxima of 650 nm and 671 nm respectively.