Labelling

BDP TMR is a borondipyrromethene dye for the TAMRA channel, analog of BODIPY® TMR. The dye has much brighter fluorescence than TAMRA. Because of the rather long lifetime of the excited state, this fluorophore is a good choice for fluorescence anisotropy measurements. This derivative is an amine-reactive NHS ester with an aminohexanoic acid (C6) linker between the dye and the functional group. The dye is suitable for labeling proteins, peptides, and other molecules with amino groups.

BDPep FL is a bright and photostable fluorophore for the fluorescein (FAM) channel. BDPep FL is an acid-stable version of BDP FL, keeping similar spectral properties to its ancestor. The compatibility of BDPep FL with TFA with additives used in solid phase peptide synthesis (SPPS) is a crucial advantage of this dye compared to other analogs. BDPep FL is beneficial for peptide synthesis due to its high stability and the absence of the dye decomposition to the corresponding dipyrrin under acidic conditions.

DusQ 1 NHS ester derivative is used for labeling with the primary amines of biomolecules. DusQ 1 is a dark quencher with an absorbance maximum of 522 nm, it can be used in qPCR probes applications, and multiplex assays. In FRET applications as an acceptor pairing with fluorophores, that emit in green to yellow range including FAM, JOE, VIC, R6G, HEX, TET.

DusQ 2 succinimidyl ester is a derivative with an amine reactive group for labeling with the amine-modified oligonucleotides, amine-containing proteins and other molecules. DusQ 2 is a nonfluorescent quencher with an absorbance maximum of 552 nm. It is useful as an acceptor in fluorescence resonance energy transfer (FRET) applications, nucleic acid assays in conjunction with yellow and orange to red emitting dyes such as Cyanine3, Cyanine5, ROX, Texas Red, AF 555, AF 594, AF 647. NHS ester function allows to conjugate the quencher to various biomolecules of interest, and also to prepare oligonucleotides with DusQ 2.

DusQ 21 is a dark quencher used as an acceptor in FRET applications, it has absorption maximum 656 nm, and can be used in multiplex assays due to the lack of secondary fluorescence. It is a quencher of choice for pairing with fluorescent dyes that emit in the red and near-infrared part of the range. DusQ 21 NHS ester derivative is used for labeling with the primary amines of amine-containing molecules. It can be used for quenching of many fluorophores, including Cyanine5, Cyanine5.5, AF 647.

DusQ 3 NHS ester is a dark, far-red nonfluorescent DusQ 3 derivative for labeling with the primary amines of proteins, modified oligonucleotides. The labeling with covalent amide bond occurs most efficiently at pH 7-9. DusQ 3 is used as an acceptor for preparation of fluorogenic dual-labeled probes, for qPCR, sequencing and other fluorescence-quenched system applications. Due to quenching range characterised as 620-730 nm DusQ 3 is useful as an acceptor in fluorescence resonance energy transfer (FRET) combined with far-red to near-IR emitting dyes such as Cyanine5, Cyanine5.5, AF 647.

HEX NHS ester, 6-isomer is an amine-reactive dye for labeling of amine groups in proteins, peptides, amino-modified oligos, and other target molecules. HEX is a hexachlorinated derivate of the fluorescent dye fluorescein. HEX exhibits an excitation peak at 533 nm and an emission peak at 549 in the green-yellow area. It can serve as a substitute for JOE, BODIPY™ 530/550, VIC™ because of their similar spectral characteristics. HEX NHS ester is widely used in nucleic acid sequencing, PCR/qPCR, and related research.

JOE is a fluorescein-derived xanthene dye containing methoxy groups and chlorine atoms. Its absorption and emission spectra are between FAM and TAMRA/ROX channels. For this reason, this fluorophore is often used for multiplex detection, including DNA sequencing. This derivative is an activated N-hydroxysuccinimide ester used to label proteins, peptides, amino-modified oligonucleotides, and other target molecules via conjugation with amines.

Thiol-reactive, near infrared dye Cyanine7.5 for the labeling of protein SH groups, an analog of Cy7.5® maleimide. Most proteins contain sulfhydryl groups which can be selectively labeled with maleimides. With this reagent, these proteins can be converted to near infrared (NIR) fluorescent conjugates. After it, the conjugates can be used in non-invasive live organism imaging to study distribution of the protein in tissues and organs.