Labelling

BP Fluor 430 hydrazide is a chemical reagent containing the BP Fluor 430 dye and a hydrazide group. Hydrazides can readily react with aldehydes such as those found on aldose-type sugars to form hydrazone bonds. BP Fluor 430 is a green fluorescent dye with excitation and emission maxima at 430 nm and 539 nm respectively.

sulfo-Cyanine5 is a sulfated derivative of Cyanine5 dye, well soluble in water because of two negatively charged sulfo groups in its structure. By its spectral characteristics, this far-red fluorescent dye is an analog of Cy™5. Hydrazides efficiently react with aldehydes and ketones resulting in hydrazones, so this compound can be used for conjugation with carbonyl derivatives of biomolecules. The reaction runs in aqueous conditions, which is important when working with antibodies and proteins. Cys-diol groups in sugars in glycosylated proteins and antibodies can be oxidized into dialdehydes, and cysteine in proteins can be converted with enzymes to formyl glycerol (i. e. reactive groups for conjugation with sulfo-Cyanine5 hydrazide). Carboxyl groups of aspartic and glutamic acids in proteins and peptides can be also conjugated with sulfo-Cyanine5 hydrazide in the presence of activating agents: carbodiimide (EDAC) or methyl morpholine (DMTMM) derivatives.

BDP 576/589, a boron dipyrromethene dye, is an analog of BODIPY™ 576/589 dye. This hydrophobic dye with emission in the orange spectrum range has high quantum yield and high molar extinction coefficient. It is suitable for experiments measuring fluorescence lifetime and fluorescence polarization. This fluorophore can be used whether in wide-field and confocal microscopy or in multiphoton microscopy because of its high two-photon absorption cross-section. This derivative is a carboxylic acid (contains a free COOH-group). It can be used as a reference control and for conjugation with an amino group after activation with carbodiimides.

DusQ 21 is a fluorescence quencher. DusQ 21 possesses broad absorption in far red and NIR range, and can quench fluorescence of dyes that emit in this region. This is a carboxylic acid derivative. DusQ 21 has intense absorption maximum at 661 nm, making it useful as an acceptor in fluorescence resonance energy transfer (FRET) applications. It is a common quencher for Cyanine5, Cyanine5.5, AF 647, or other spectrally similar fluorescent dyes.

BP Fluor 488 is a stable dye over a broad pH range (from 4 to 10) with the fluorescence peak in the green region (at 519 nm). Conjugates of biomolecules with BP Fluor 488 can be used in flow cytometry, for example in multicolor staining. BP Fluor 488 is also used in cell microscopy due to its high photostability. BP Fluor 488 amine is soluble in water and can be conjugated with electrophiles and participate in enzymatic transamination.

Cyanine7.5 is a NIR fluorescent dye. This reagent contains free amino group which can be used coupled with activated carboxylic acid derivatives.

This product is an amine derivative of ROX (Rhodamine X, Rhodamine 101) dye with an amine group separated from the fluorophore by a long linker that facilitates conjugation. The aliphatic primary amine group can be coupled with various electrophiles (activated esters, epoxides, etc.) and used in enzymatic transamination labeling. ROX is a red-emitting fluorophore possessing high brightness and fluorescence quantum yield. This reagent is a pure 6-isomer.

Cyanine3.5 dimethyl is a non-sulfonated cyanine dye with good solubility in organic solvents and bright fluorescence in the yellow-orange spectrum range. The dye can be used as a non-reactive fluorophore for control experiments, calibration, and other technical applications.

Non-functionalized Cyanine7.5 dye derivative with an aliphatic side chain and good solubility in organic solvents. The dye can be used as a non-reactive fluorophore for control experiments and for calibration.