Buffers & Concentrates

D-Luciferin K salt is a firefly luciferase substrate.

CUTANA™ Nuclei Extraction Buffer is the essential reagent for harvesting nuclei from cultured cells and tissues for use in CUT&RUN and CUT&Tag assays. This buffer is expected to be broadly compatible with eukaryotic cells and tissues. Below are examples of various cells and tissue types that have undergone successful nuclei isolation using this buffer: mouse NIH3T3 fibroblast cells human A549 non-small cell lung cancer (NSCLC) cells human K562 leukemia cells human NCI-H1299 non-small cell lung cancer (NSCLS cells) human bone marrow derived macrophages human TIG-1 fetal lung cells human monocyte derived macrophages human LoVo colorectal cancer cells human MV-4-11 macrophage cells human LNCaP prostate carcinoma cells human SUM149 triple negative breast cancer (TNBC) cells human renal primary cells human GM24385 B-lymphocyte (aka HG002) cells human peripheral blood mononuclear cells (PBMCs) human MCF7 breast cancer cells human intestinal tissue human MDA-MB-231 bre

PEG Blocking Buffer for ELISA contain polyoxyethylene detergents that interfere with PEG and PEG antibody detection. PEGB125-1 effectively blocks and stabilizes PEG-protein and anti-PEG coated ELISA plates if used as directed.

PEG Dilution Buffer contains an effective blocking agent that minimizes non specific binding and a non polyoxyethylene detergent that decreases variability and background.

PEG Wash Buffer contains a non polyoxyethylene detergent that decreases variability and background.

Mammalian Cell Lysis Buffer (5X) requires minimal hands-on time and only needs a simple 5-fold dilution.

This product is a pre-cast gel for electrophoresis made with polyacrylamide. The Phos-tag contained in a gel during SDS-PAGE supplements the phosphoproteins, retarding the electrophoretic velocity of the phosphoproteins. In this way, the phosphoproteins and unphosphorylated proteins can be detected as separate bands.

 precast polyacrylamide gels containing Phos-tag for SDS-PAGE