Lentivirus

Membrane eGFP Lentivirus are replication incompetent, HIV based, VSV-G pseudotyped lentiviral particles that are ready to transduce almost all types of mammalian cells, including primary and non-dividing cells. These particles contain eGFP (enhanced green fluorescent protein), with a plasma membrane-targeting sequence at the N-terminus of eGFP, under the control of an EF1a promoter. The lentiviruses also transduce a puromycin selection marker. eGFP expression and transduction efficiency can easily be verified and optimized via fluorescence microscopy or flow cytometry.

Mitochondrial eGFP Lentivirus are replication incompetent, HIV based, VSV-G pseudotyped lentiviral particles that are ready to transduce almost all types of mammalian cells, including primary and non-dividing cells. These particles contain eGFP (enhanced green fluorescent protein), with a mitochondrial targeting sequence at the N-terminus of eGFP), under the control of an EF1a promoter. The lentiviruses also transduce a puromycin selection marker. eGFP expression and transduction efficiency can easily be verified and optimized via fluorescence microscopy or flow cytometry.

Endoplasmic Reticulum (ER) eGFP Lentivirus are replication incompetent, HIV based, VSV-G pseudotyped lentiviral particles that are ready to transduce almost all types of mammalian cells, including primary and non-dividing cells. These particles contain eGFP (enhanced green fluorescent protein), with an ER-targeting and retrieval sequences at both the N and C-terminus of eGFP, respectively, under the control of an EF1a promoter. The lentiviruses also transduce a puromycin selection marker (Figure 1). eGFP expression and transduction efficiency can easily be verified and optimized via fluorescence microscopy or flow cytometry.

EGFP Beta-Actin Lentivirus are replication incompetent, HIV based, VSV-G pseudotyped lentiviral particles that are ready to transduce almost all types of mammalian cells, including primary and non-dividing cells. These particles contain eGFP and β-actin (NM_001101.5) (eGFP-Linker-β-actin) driven by an EF1A promoter. The lentiviruses also transduce a puromycin selection marker (Figure 1). eGFP expression and transduction efficiency can easily be verified and optimized via fluorescence microscopy or flow cytometry.

eGFP-Tubulin Lentivirus are replication incompetent, HIV based, VSV-G pseudotyped lentiviral particles that are ready to transduce almost all types of mammalian cells, including primary and non-dividing cells. These particles contain eGFP and α-tubulin (eGFP-linker-alpha-tubulin) driven by an EF1A promoter. The lentiviruses also transduce a puromycin selection marker. eGFP expression and transduction efficiency can easily be verified and optimized via fluorescence microscopy or flow cytometry.

Nuclear mCherry Lentivirus are replication incompetent, HIV based, VSV-G pseudotyped lentiviral particles that are ready to transduce almost all types of mammalian cells, including primary and non-dividing cells. These particles contain a nuclear mCherry, with nuclear localization sequences (NLS) at both the N- and C-terminus of mCherry, under the control of an EF1a promoter. The lentiviruses also transduce a puromycin selection marker. mCherry expression and transduction efficiency can easily be verified and optimized via fluorescence microscopy or flow cytometry.

The Firefly Luciferase-Nuclear eGFP Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to transduce almost all types of mammalian cells, including primary and non-dividing cells. These particles contain firefly luciferase and nuclear eGFP (Luc2-P2A-nuclear eGFP) driven by an EF1A promoter. These lentiviruses also transduce a puromycin selection marker (Figure 1). The nuclear eGFP has NLS sequences at the C-terminus. eGFP has an excitation wavelength of 488 nm, an emission wavelength of 509 nm, and extinction coefficient of 55,000 M-1cm-1.

The NFĸB Luciferase-eGFP Reporter Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to transduce almost all types of mammalian cells, including primary and nondividing cells. The particles contain a firefly luciferase and eGFP (enhanced green fluorescent protein) cassette driven by the NFĸB (nuclear factor kappa-light-chain-enhancer of activated B cells) response element located upstream of the minimal TATA promoter. These lentiviruses also transduce a puromycin selection marker (Figure 1). After transduction, activation of the NFĸB signaling pathway in the target cells can be monitored by measuring luciferase activity or/and eGFP expression.

The CDH17 Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles ready to transduce almost all types of mammalian cells, including primary and non-dividing cells. These viruses transduce cells with human CDH17 (cadherin 17) (NM_004063.4) driven by a CMV promoter. The lentiviruses also transduce a puromycin selection marker.