Results for Labelling ( 3144 )
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TCO (tetrazine – trans-cyclooctene) ligation is one of the fastest reactions used for bioconjugation. It is an inverse electron demand [4+2] cycloaddition that takes place between tetrazine and trans-cyclooctene or other strained olefins. sulfo-Cyanine5 tetrazine is a fluorophore derivative bearing a tetrazine group for the TCO-ligation-based labeling. This reagent possesses good aqueous solubility and stability in biological environments.
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sulfo-Cyanine5.5 is a water-soluble, hydrophilic far-red fluorophore, an analog of Cy5.5®. Like other cyanine dyes, sulfo-Cyanine5.5 has an outstanding molar extinction coefficient, giving rise to its bright fluorescence. The molecule contains four sulfo groups that provide hydrophilicity and negative charge to the fluorophore and minimize non-specific binding. This reagent contains tetrazine moiety that reacts with trans-cyclooctenes, cyclopropenes, and some strained cyclooctynes to form stable conjugates via an inverted electron demand [4+2]-cycloaddition (IEDDA). The reagent possesses high aqueous solubility and hydrophilicity and is recommended for labeling biomolecules in an aqueous environment.
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Dibenzocyclooctyne (DBCO, DBCO, ADIBO) is one of the most reactive cycloalkynes for copper-free click reaction (SPAAC, strain-promoted azide-alkyne cycloaddition). The rate of interaction of DBCO with azides is significantly higher than that of other cyclooctynes, as well as Cu-catalyzed click reaction (CuAAC). Unlike other cyclooctynes, DBCO does not interact with tetrazines, which makes it possible to use it in bioorthogonal reactions together with trans-cyclooctenes and tetrazines. BP Fluor 568 is a bright, photostable, and hydrophilic fluorophore that emits in the orange channel. The absorption maximum is 572 nm. The emission maximum is 598 nm. BP Fluor 568 DBCO allows fluorescent labeling of azide-containing biomolecules inside living cells and whole organisms without the negative effect of copper ions on them, and inanimate samples.
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Strain promoted alkyne azide cycloaddition (SPAAC) between strained cycloalkynes and azides is a useful bioconjugation method that eliminates the need for copper catalyst necessary for classical CuAAC click chemistry. Cyanine5.5 DBCO is a cyclooctyne product containing Cyanine5.5 fluorescent dye. This fluorophore is used in bioimaging, biodistribution studies, and microscopy. Cyanine5.5 DBCO can be used to conjugate the fluorophore with various azides.
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This product is a derivative of tetramethylrhodamine (TMR, TAMRA) containing a cyclooctyne moiety (dibenzocyclooctyne, DBCO). Pure 5-isomer. DBCO reacts quickly and efficiently with azides by simply mixing the components without the need for a copper catalyst (so-called sterically promoted cycloaddition reaction (SPAAC)). TAMRA DBCO can be used for the labeling of proteins, peptides, nucleic acids, and other molecules containing azide groups. TAMRA is often used as a FRET acceptor for FAM fluorophore. Can replace DyLight 549.
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Copper-free click chemistry reaction between strained cycloalkynes (cyclooctynes), and azides, is a very fast and robust reaction. It can be used for a fast labeling with fluorescent dyes. This reagent is a derivative of water soluble sulfo-Cyanine5 dye which emits in red channel. It is useful for the labeling of biomolecules in aqueous media.