Cell-based Assays

Measure real-time cAMP signaling kinetics in the primary cilia with this live cell fluorescent assay. Add 5HT6-tagged cADDis to your cells and measure robust signals the next day on a fluorescence imaging system. Decreasing green fluorescence indicates ciliary cAMP increase. This assay is genetically encoded and packaged in BacMam for efficient transduction in most cell types - including uniform and near 100% efficiency in HEK293 cells as well as expression in many primary or iPSC-derived cells. The kit includes the cilia-targeted cAMP sensor BacMam and 500mM sodium butyrate in H2O. 30mL size is available as well.

Measure real-time cAMP signaling kinetics in the nucleus cilia with this live cell fluorescent assay. Add NLS-tagged cADDis to your cells and measure robust signals the next day on a fluorescence imaging system. Decreasing green fluorescence indicates cAMP increase. This assay is genetically encoded and packaged in BacMam for efficient transduction in most cell types - including uniform and near 100% efficiency in HEK293 cells as well as expression in many primary or iPSC-derived cells. The kit includes the cAMP sensor BacMam, 500mM sodium butyrate in H2O, and a positive control receptor and agonist (B2AR BacMam and 10mM isoproterenol in HCl). 30mL size is available as well. Other Montana Molecular tools including BacMam-packaged GPCRs, red fluorescent membrane-targeted cAMP sensors, or red fluorescent sensors for DAG or Calcium can easily be co-expressed with this assay.

Measure real-time cAMP signaling kinetics with this flag-tagged MyrPalm cADDis cAMP sensor. Add fMP-tagged cADDis to your cells and measure robust signals the next day on a fluorescence imaging system. Decreasing green fluorescence indicates cAMP increase. This assay is genetically encoded and packaged in BacMam for efficient transduction in most cell types - including uniform and near 100% efficiency in HEK293 cells as well as expression in many primary or iPSC-derived cells. The kit includes the cAMP sensor BacMam, 500mM sodium butyrate in H2O, and a positive control receptor and agonist (B2AR BacMam and 10mM isoproterenol in HCl). 30mL size is available as well. Other Montana Molecular tools including BacMam-packaged GPCRs, red fluorescent membrane-targeted or cytosolic cAMP sensors, or red fluorescent sensors for DAG or Calcium can easily be co-expressed with this assay.

Measure diacylglycerol (DAG) signaling kinetics, screen GPCRs for Gq activity, and more with this live cell fluorescent assay. Add the DAG sensor to your cells and measure robust signals the next day on an automated plate reader or fluorescence imaging system. Decreasing green fluorescence indicates DAG increase. These assays are genetically encoded and packaged in BacMam for efficient transduction in most cell types - including uniform and near 100% efficiency in HEK293 cells as well as expression in many primary or iPSC-derived cells. The kit includes the DAG sensor BacMam, 500mM sodium butyrate in H2O, and a positive control receptor and agonist (hM1R BacMam and 25mM carbachol in H2O). 30mL and 60mL sizes are available as well. Other Montana Molecular tools including BacMam-packaged GPCRs can easily be co-expressed with this assay.

Measure diacylglycerol (DAG) signaling kinetics, screen GPCRs for Gq activity, and more with this live cell fluorescent assay. Add the DAG sensor to your cells and measure robust signals the next day on an automated plate reader or fluorescence imaging system. Decreasing green fluorescence indicates DAG increase. These assays are genetically encoded and packaged in BacMam for efficient transduction in most cell types - including uniform and near 100% efficiency in HEK293 cells as well as expression in many primary or iPSC-derived cells. The kit includes the DAG sensor BacMam, 500mM sodium butyrate in H2O, and a positive control receptor and agonist (hM1R BacMam and 25mM carbachol in H2O). 10mL and 60mL sizes are available as well. Other Montana Molecular tools including BacMam-packaged GPCRs can easily be co-expressed with this assay.

Measure diacylglycerol (DAG) signaling kinetics, screen GPCRs for Gq activity, and more with this live cell fluorescent assay. Add the DAG sensor to your cells and measure robust signals the next day on an automated plate reader or fluorescence imaging system. Decreasing green fluorescence indicates DAG increase. These assays are genetically encoded and packaged in BacMam for efficient transduction in most cell types - including uniform and near 100% efficiency in HEK293 cells as well as expression in many primary or iPSC-derived cells. The kit includes the DAG sensor BacMam, 500mM sodium butyrate in H2O, and a positive control receptor and agonist (hM1R BacMam and 25mM carbachol in H2O). 10mL and 30mL sizes are available as well. Other Montana Molecular tools including BacMam-packaged GPCRs can easily be co-expressed with this assay.

Measure PIP2 signaling kinetics, screen GPCRs for Gq activity, and more with this live cell fluorescent assay. Add the PIP2 sensor to your cells and measure robust signals the next day on an automated plate reader or fluorescence imaging system. These assays are genetically encoded and packaged in BacMam for efficient transduction in most cell types - including uniform and near 100% efficiency in HEK293 cells as well as expression in many primary or iPSC-derived cells. The kit includes the PIP2 sensor BacMam, 500mM sodium butyrate in H2O, and a positive control receptor and agonist (hM1R BacMam and 25mM carbachol in H2O). 30mL and 60mL sizes are available as well. Other Montana Molecular tools including BacMam-packaged GPCRs can easily be co-expressed with this assay.

Measure PIP2 signaling kinetics, screen GPCRs for Gq activity, and more with this live cell fluorescent assay. Add the PIP2 sensor to your cells and measure robust signals the next day on an automated plate reader or fluorescence imaging system. These assays are genetically encoded and packaged in BacMam for efficient transduction in most cell types - including uniform and near 100% efficiency in HEK293 cells as well as expression in many primary or iPSC-derived cells. The kit includes the PIP2 sensor BacMam, 500mM sodium butyrate in H2O, and a positive control receptor and agonist (hM1R BacMam and 25mM carbachol in H2O). 10mL and 60mL sizes are available as well. Other Montana Molecular tools including BacMam-packaged GPCRs can easily be co-expressed with this assay.

Measure PIP2 signaling kinetics, screen GPCRs for Gq activity, and more with this live cell fluorescent assay. Add the PIP2 sensor to your cells and measure robust signals the next day on an automated plate reader or fluorescence imaging system. These assays are genetically encoded and packaged in BacMam for efficient transduction in most cell types - including uniform and near 100% efficiency in HEK293 cells as well as expression in many primary or iPSC-derived cells. The kit includes the PIP2 sensor BacMam, 500mM sodium butyrate in H2O, and a positive control receptor and agonist (hM1R BacMam and 25mM carbachol in H2O). 10mL and 30mL sizes are available as well. Other Montana Molecular tools including BacMam-packaged GPCRs can easily be co-expressed with this assay.