Cell-based Assays

Measure PIP2 signaling kinetics, screen GPCRs for Gq activity, and more with this live cell fluorescent assay. Add the PIP2 sensor to your cells and measure robust signals in 30 hours on fluorescence imaging systems. These assays are genetically encoded and packaged in BacMam for efficient transduction in most cell types - including uniform and near 100% efficiency in HEK293 cells as well as expression in many primary or iPSC-derived cells. The kit includes the PIP2 sensor BacMam, 500mM sodium butyrate in H2O, and a positive control receptor and agonist (hM1R BacMam and 25mM carbachol in H2O). Other Montana Molecular tools including BacMam-packaged GPCRs can easily be co-expressed with this assay.

Measure cyclic GMP (cGMP) signaling kinetics, examine PDE biology, ascertain the specificity of PDE inhibitors, and more with the live cell, fluorescent, GENIe cGMP assay. Add the GENIe sensor to your cells and measure robust signals the next day on an automated plate reader or fluorescence imaging system. Decreasing green fluorescence indicates cGMP increase. This assay is genetically encoded and packaged in BacMam for efficient transduction in most cell types - including uniform and near 100% efficiency in HEK293 cells as well as expression in many primary or iPSC-derived cells. The kit includes the cGMP sensor BacMam, soluble guanylate cyclase BacMam, and 500mM sodium butyrate in H2O. Other Montana Molecular tools including the cADDis cAMP sensor can be used alongside this assay.

Measure cyclic AMP (cAMP) signaling kinetics, screen GPCRs for Gs activity, and more with the live cell, fluorescent, cADDis cAMP assay. Add the cADDis sensor to your cells and measure robust signals the next day on an automated plate reader or fluorescence imaging system. Increasing green fluorescence indicates cAMP increase. This assay is genetically encoded and packaged in BacMam for efficient transduction in most cell types - including uniform and near 100% efficiency in HEK293 cells as well as expression in many primary or iPSC-derived cells. The kit includes the cAMP sensor BacMam, 500mM sodium butyrate in H2O, and a positive control receptor and agonist (B2AR BacMam and 10mM isoproterenol in HCl). 30mL and 60mL sizes are available as well. Other Montana Molecular tools including BacMam-packaged GPCRs or red fluorescent DAG and Calcium sensors can easily be co-expressed with this assay.

Measure cyclic AMP (cAMP) signaling kinetics, screen GPCRs for Gs activity, and more with the live cell, fluorescent, cADDis cAMP assay. Add the cADDis sensor to your cells and measure robust signals the next day on an automated plate reader or fluorescence imaging system. Increasing red fluorescence indicates cAMP increase. This assay is genetically encoded and packaged in BacMam for efficient transduction in most cell types - including uniform and near 100% efficiency in HEK293 cells as well as expression in many primary or iPSC-derived cells. The kit includes the cAMP sensor BacMam, 500mM sodium butyrate in H2O, and a positive control receptor and agonist (B2AR BacMam and 10mM isoproterenol in HCl). 30mL and 60mL sizes are available as well. Other Montana Molecular tools including BacMam-packaged GPCRs or green fluorescent ER stress and DAG sensors can easily be co-expressed with this assay.

Measure cyclic AMP (cAMP) signaling kinetics, screen GPCRs for Gs activity, and more with the live cell, fluorescent, cADDis cAMP assay. Add the cADDis sensor to your cells and measure robust signals the next day on an automated plate reader or fluorescence imaging system. Increasing red fluorescence indicates cAMP increase. This assay is genetically encoded and packaged in BacMam for efficient transduction in most cell types - including uniform and near 100% efficiency in HEK293 cells as well as expression in many primary or iPSC-derived cells. The kit includes the cAMP sensor BacMam, 500mM sodium butyrate in H2O, and a positive control receptor and agonist (B2AR BacMam and 10mM isoproterenol in HCl). 10mL and 60mL sizes are available as well. Other Montana Molecular tools including BacMam-packaged GPCRs or green fluorescent ER stress and DAG sensors can easily be co-expressed with this assay.

Measure cyclic AMP (cAMP) signaling kinetics, screen GPCRs for Gs activity, and more with the live cell, fluorescent, cADDis cAMP assay. Add the cADDis sensor to your cells and measure robust signals the next day on an automated plate reader or fluorescence imaging system. Increasing red fluorescence indicates cAMP increase. This assay is genetically encoded and packaged in BacMam for efficient transduction in most cell types - including uniform and near 100% efficiency in HEK293 cells as well as expression in many primary or iPSC-derived cells. The kit includes the cAMP sensor BacMam, 500mM sodium butyrate in H2O, and a positive control receptor and agonist (B2AR BacMam and 10mM isoproterenol in HCl). 10mL and 30mL sizes are available as well. Other Montana Molecular tools including BacMam-packaged GPCRs or green fluorescent Cell Stress and Calcium sensors can easily be co-expressed with this assay.

Measure real-time cAMP signaling kinetics in the nucleus cilia with this live cell fluorescent assay. Add NLS-tagged cADDis to your cells and measure robust signals the next day on a fluorescence imaging system. Increasing red fluorescence indicates cAMP increase. This assay is genetically encoded and packaged in BacMam for efficient transduction in most cell types - including uniform and near 100% efficiency in HEK293 cells as well as expression in many primary or iPSC-derived cells. The kit includes the cAMP sensor BacMam, 500mM sodium butyrate in H2O, and a positive control receptor and agonist (B2AR BacMam and 10mM isoproterenol in HCl). 30mL size is available as well. Other Montana Molecular tools including BacMam-packaged GPCRs, green fluorescent membrane-targeted or cytosolic cAMP sensors, or green fluorescent sensors for DAG or Calcium can easily be co-expressed with this assay.

Measure real-time cAMP signaling kinetics with this flag-tagged S15 cADDis cAMP sensor. Add fS15-tagged cADDis to your cells and measure robust signals the next day on a fluorescence imaging system. Increasing red fluorescence indicates cAMP increase. This assay is genetically encoded and packaged in BacMam for efficient transduction in most cell types - including uniform and near 100% efficiency in HEK293 cells as well as expression in many primary or iPSC-derived cells. The kit includes the cAMP sensor BacMam, 500mM sodium butyrate in H2O, and a positive control receptor and agonist (B2AR BacMam and 10mM isoproterenol in HCl). 30mL size is available as well. Other Montana Molecular tools including BacMam-packaged GPCRs, green fluorescent nucleus-targeted or cytosolic cAMP sensors, or green fluorescent sensors for DAG or PIP2 can easily be co-expressed with this assay.

Measure diacylglycerol (DAG) signaling kinetics, screen GPCRs for Gq activity, and more with this live cell fluorescent assay. Add the DAG sensor to your cells and measure robust signals the next day on an automated plate reader or fluorescence imaging system. Increasing green fluorescence indicates DAG increase. These assays are genetically encoded and packaged in BacMam for efficient transduction in most cell types - including uniform and near 100% efficiency in HEK293 cells as well as expression in many primary or iPSC-derived cells. The kit includes the DAG sensor BacMam, 500mM sodium butyrate in H2O, and a positive control receptor and agonist (hM1R BacMam and 25mM carbachol in H2O). 30mL and 60mL sizes are available as well. Other Montana Molecular tools including BacMam-packaged GPCRs can easily be co-expressed with this assay.