Cell-based Assays

Measure diacylglycerol (DAG) signaling kinetics, screen GPCRs for Gq activity, and more with this live cell fluorescent assay. Add the DAG sensor to your cells and measure robust signals the next day on an automated plate reader or fluorescence imaging system. Increasing green fluorescence indicates DAG increase. These assays are genetically encoded and packaged in BacMam for efficient transduction in most cell types - including uniform and near 100% efficiency in HEK293 cells as well as expression in many primary or iPSC-derived cells. The kit includes the DAG sensor BacMam, 500mM sodium butyrate in H2O, and a positive control receptor and agonist (hM1R BacMam and 25mM carbachol in H2O). 10mL and 60mL sizes are available as well. Other Montana Molecular tools including BacMam-packaged GPCRs can easily be co-expressed with this assay.

Measure diacylglycerol (DAG) signaling kinetics, screen GPCRs for Gq activity, and more with this live cell fluorescent assay. Add the DAG sensor to your cells and measure robust signals the next day on an automated plate reader or fluorescence imaging system. Increasing green fluorescence indicates DAG increase. These assays are genetically encoded and packaged in BacMam for efficient transduction in most cell types - including uniform and near 100% efficiency in HEK293 cells as well as expression in many primary or iPSC-derived cells. The kit includes the DAG sensor BacMam, 500mM sodium butyrate in H2O, and a positive control receptor and agonist (hM1R BacMam and 25mM carbachol in H2O). 30mL and 60mL sizes are available as well. Other Montana Molecular tools including BacMam-packaged GPCRs can easily be co-expressed with this assay.

Measure diacylglycerol (DAG) signaling kinetics, screen GPCRs for Gq activity, and more with this live cell fluorescent assay. Add the DAG sensor to your cells and measure robust signals after 30 hours on an automated plate reader or fluorescence imaging system. Increasing red fluorescence indicates DAG increase. These assays are genetically encoded and packaged in BacMam for efficient transduction in most cell types - including uniform and near 100% efficiency in HEK293 cells as well as expression in many primary or iPSC-derived cells. The kit includes the DAG sensor BacMam, 500mM sodium butyrate in H2O, and a positive control receptor and agonist (hM1R BacMam and 25mM carbachol in H2O). 30mL size is available as well. Other Montana Molecular tools including BacMam-packaged GPCRs can easily be co-expressed with this assay.

Measure diacylglycerol (DAG) signaling kinetics, screen GPCRs for Gq activity, and more with this live cell fluorescent assay. Add the DAG sensor to your cells and measure robust signals after 30 hours on an automated plate reader or fluorescence imaging system. Increasing red fluorescence indicates DAG increase. These assays are genetically encoded and packaged in BacMam for efficient transduction in most cell types - including uniform and near 100% efficiency in HEK293 cells as well as expression in many primary or iPSC-derived cells. The kit includes the DAG sensor BacMam, 500mM sodium butyrate in H2O, and a positive control receptor and agonist (hM1R BacMam and 25mM carbachol in H2O). 10mL size is available as well. Other Montana Molecular tools including BacMam-packaged GPCRs can easily be co-expressed with this assay.

Measure Calcium signaling kinetics, screen GPCRs for Gq activity, and more with this live cell fluorescent assay. Add the red fluorescent R-GECO Calcium sensor to your cells and measure robust signals after 30 hours on an automated plate reader or fluorescence imaging system. This assay is genetically encoded and packaged in BacMam for efficient transduction in most cell types - including uniform and near 100% efficiency in HEK293 cells as well as expression in many primary or iPSC-derived cells. The kit includes the R-GECO sensor BacMam, 500mM sodium butyrate in H2O, and a positive control receptor and agonist (hM1R BacMam and 25mM carbachol in H2O). 30mL and 60mL sizes are available as well. Other Montana Molecular tools including BacMam-packaged GPCRs or ion channels can easily be co-expressed with this assay.

Measure Calcium signaling kinetics, screen GPCRs for Gq activity, and more with this live cell fluorescent assay. Add the red fluorescent R-GECO Calcium sensor to your cells and measure robust signals after 30 hours on an automated plate reader or fluorescence imaging system. This assay is genetically encoded and packaged in BacMam for efficient transduction in most cell types - including uniform and near 100% efficiency in HEK293 cells as well as expression in many primary or iPSC-derived cells. The kit includes the R-GECO sensor BacMam, 500mM sodium butyrate in H2O, and a positive control receptor and agonist (hM1R BacMam and 25mM carbachol in H2O). 10mL and 60mL sizes are available as well. Other Montana Molecular tools including BacMam-packaged GPCRs or ion channels can easily be co-expressed with this assay.

Detect chemical or genetic stress, and identify compounds that reverse the stress response with this live cell fluorescent assay. Measure ER stress responses via the XBP1/IRE1 arm of the UPR. Add the cell stress sensor to your cells and measure robust signals the next day on a fluorescence imaging system or automated plate reader. Increasing green fluorescence indicates ER Stress or UPR activation. Constitutively expressed red fluorescent protein can be used to identify transduced cells. This assay is genetically encoded and packaged in BacMam for efficient transduction in most cell types - including uniform and near 100% efficiency in HEK293 cells as well as expression in many primary or iPSC-derived cells. The kit includes the ER stress sensor BacMam, 500mM sodium butyrate in H2O, and a positive control agonist (1mM thapsigargin in DMSO). 10mL and 60mL sizes are available as well. Other Montana Molecular tools including BacMam-packaged proteins associated with neurodegeneration like

Detect chemical or genetic stress, and identify compounds that reverse the stress response with this live cell fluorescent assay. Measure ER stress responses via the XBP1/IRE1 arm of the UPR. Add the cell stress sensor to your cells and measure robust signals the next day on a fluorescence imaging system or automated plate reader. Increasing green fluorescence indicates ER Stress or UPR activation. Constitutively expressed red fluorescent protein can be used to identify transduced cells. This assay is genetically encoded and packaged in BacMam for efficient transduction in most cell types - including uniform and near 100% efficiency in HEK293 cells as well as expression in many primary or iPSC-derived cells. The kit includes the ER stress sensor BacMam, 500mM sodium butyrate in H2O, and a positive control agonist (1mM thapsigargin in DMSO). 10mL and 30mL sizes are available as well. Other Montana Molecular tools including BacMam-packaged proteins associated with neurodegeneration like

Measure cyclic AMP (cAMP) signaling kinetics, screen GPCRs for Gi activity, and more with the live cell, fluorescent, cADDis cAMP assay. Add the cADDis sensor to your cells and measure robust signals the next day on an automated plate reader or fluorescence imaging system. Increasing green fluorescence indicates cAMP decrease. This assay is genetically encoded and packaged in BacMam for efficient transduction in most cell types - including uniform and near 100% efficiency in HEK293 cells as well as expression in many primary or iPSC-derived cells. The kit includes the cAMP sensor BacMam, 500mM sodium butyrate in H2O, and a positive control receptor and agonist (D2R BacMam and 2mM quinpirole in H2O). 30mL and 60mL sizes are available as well. Other Montana Molecular tools including BacMam-packaged GPCRs can easily be co-expressed with this assay.