Cell-based Assays

Measure arrestin signaling kinetics in real time with the live cell, fluorescent Borealis arrestin - PAR2 assay. Add the unmodified PAR2 receptor and Borealis sensor to cells and measure robust signals the next day on an automated plate reader or fluorescence imaging system. This assay is genetically encoded and packaged in BacMam for efficient transduction in most cell types - including uniform and near 100% efficiency in HEK293 cells as well as expression in many primary or iPSC-derived cells. The kit includes the arrestin sensor BacMam, PAR2 BacMam, 500mM sodium butyrate in H2O, and a positive control receptor and agonist (AT1R BacMam and 5mM Angiotensin II in H2O). 30mL and 60mL sizes are available as well, as are Borealis kits for several other receptors. Other Montana Molecular tools including the DAG and Calcium sensors can be used alongside this assay.

Measure arrestin signaling kinetics in real time with the live cell, fluorescent Borealis arrestin - Delta Opioid receptor (DOR) assay. Add the unmodified DOR and Borealis sensor to cells and measure robust signals the next day on an automated plate reader or fluorescence imaging system. This assay is genetically encoded and packaged in BacMam for efficient transduction in most cell types - including uniform and near 100% efficiency in HEK293 cells as well as expression in many primary or iPSC-derived cells. The kit includes the arrestin sensor BacMam, DOR BacMam, GRK5 BacMam, 500mM sodium butyrate in H2O, and a positive control receptor and agonist (AT1R BacMam and 5mM Angiotensin II in H2O). 30mL and 60mL sizes are available as well, as are Borealis kits for several other receptors. Other Montana Molecular tools including the cADDis cAMP sensor can be used alongside this assay.

Measure arrestin signaling kinetics in real time with the live cell, fluorescent Borealis arrestin - LPA1 receptor assay. Add the unmodified LPA1 receptor and Borealis sensor to cells and measure robust signals the next day on an automated plate reader or fluorescence imaging system. This assay is genetically encoded and packaged in BacMam for efficient transduction in most cell types - including uniform and near 100% efficiency in HEK293 cells as well as expression in many primary or iPSC-derived cells. The kit includes the arrestin sensor BacMam, LPA1R BacMam, 500mM sodium butyrate in H2O, and a positive control receptor and agonist (AT1R BacMam and 5mM Angiotensin II in H2O). 30mL and 60mL sizes are available as well, as are Borealis kits for several other receptors. Other Montana Molecular tools including the cADDis cAMP and DAG sensors can be used alongside this assay.

Measure arrestin signaling kinetics in real time with the live cell, fluorescent Borealis arrestin - PTH1 receptor assay. Add the unmodified PTH1 receptor and Borealis sensor to cells and measure robust signals the next day on an automated plate reader or fluorescence imaging system. This assay is genetically encoded and packaged in BacMam for efficient transduction in most cell types - including uniform and near 100% efficiency in HEK293 cells as well as expression in many primary or iPSC-derived cells. The kit includes the arrestin sensor BacMam, PTH1R BacMam, 500mM sodium butyrate in H2O, and a positive control receptor and agonist (AT1R BacMam and 5mM Angiotensin II in H2O). 30mL and 60mL sizes are available as well, as are Borealis kits for several other receptors. Other Montana Molecular tools including the cADDis cAMP sensor can be used alongside this assay.

This kit is designed to quantify collagen in various sources such as cell media, ECM (Extra Cellular Matrix) of culture cell and tissue because the kit detects atelo-collagen which is prepared by pepsin digestion. Sensitivity : 0.02ug/ml, Range: 0.02-4

Abbkine Annexin V-EGFP/PI Apoptosis Detection kit allows the discrimination of intact cells, early apoptotic and late apoptotic or necrotic cells.

TraKine™ Pro Live-cell Tubulin-traker kit (Red Fluorescence) is a fluorescence imaging tool for staining of Tubulin in mammalian living and fixed cells with high specificity and low background.

TraKine™ Pro Live-cell Lyso-traker kit (Green Fluorescence) is a fluorescence imaging tool for staining of Lysosome in mammalian living and fixed cells with high specificity and low background.

TraKine™ Pro Live-cell Lyso-traker kit (Red Fluorescence) is a fluorescence imaging tool for staining of Lysosome in mammalian living and fixed cells with high specificity and low background.