Cell-based Assays

Green Ca2+ indicator with CMV promoter. The fluorescent, genetically-encoded Ca2+ indicator (GECO) was developed by Robert Campbell’s lab at University of Alberta. GECO can be paired with cADDis, our red fluorescent cAMP sensor, or our red fluorescent Upward and Downward DAG sensors to simultaneously detect both Ca2+ and cyclic AMP or Ca2+ and diacylglycerol in living cells. The BacMam delivery system enables consistent and controllable expression in a wide variety of cell types including iPSC-derived cardiomyocytes and neurons. Materials in the Kit: • Green GECO Sensor BacMam • Sodium Butyrate 500 mM in H2O. • M1 muscarinic acetylcholine receptor BacMam with nuclear red label in TNM-FH Insect Culture Medium. • Carbachol 25 mM in H2O

Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-BTK (Y223) and Total BTK in cell lysates. This unique kit allows to simultaneously detect phosphorylated and total BTK proteins from the same lysate sample in separate wells

Sandwich TR-FRET immunoassay for quantitative detection of human CCL4/MIP-1β (hCCL4; MIP-1β) in cell culture supernatants. The kit does not include a recombinant human CCL4/MIP-1β standard, which must be purchased separately from R&D Systems (Catalog number 8228-MB-025).

Sandwich TR-FRET immunoassay for quantitative detection of human CCL4/MIP-1β (hCCL4; MIP-1β) in cell culture supernatants. The kit does not include a recombinant human CCL4/MIP-1β standard, which must be purchased separately from R&D Systems (Catalog number 8228-MB-025).

Sandwich TR-FRET immunoassay for quantitative detection of human CCL4/MIP-1β (hCCL4; MIP-1β) in cell culture supernatants. The kit does not include a recombinant human CCL4/MIP-1β standard, which must be purchased separately from R&D Systems (Catalog number 8228-MB-025).

Sandwich TR-FRET immunoassay for quantitative detection of human CCL4/MIP-1β (hCCL4; MIP-1β) in cell culture supernatants. The kit does not include a recombinant human CCL4/MIP-1β standard, which must be purchased separately from R&D Systems (Catalog number 8228-MB-025).

Sandwich TR-FRET immunoassay for quantitative detection of human CCL4/MIP-1β (hCCL4; MIP-1β) in cell culture supernatants. The kit does not include a recombinant human CCL4/MIP-1β standard, which must be purchased separately from R&D Systems (Catalog number 8228-MB-025).

Sandwich TR-FRET immunoassay for quantitative detection of human GM-CSF (hGM-CSF) in cell culture supernatants. The kit does not include a recombinant human GM-CSF standard, which must be purchased separately from R&D Systems (catalogue number 7954-GM).