Cell-based Assays

Measure real-time cAMP signaling kinetics in the nucleus cilia with this live cell fluorescent assay. Add NLS-tagged cADDis to your cells and measure robust signals the next day on a fluorescence imaging system. Increasing red fluorescence indicates cAMP increase. This assay is genetically encoded and packaged in BacMam for efficient transduction in most cell types - including uniform and near 100% efficiency in HEK293 cells as well as expression in many primary or iPSC-derived cells. The kit includes the cAMP sensor BacMam, 500mM sodium butyrate in H2O, and a positive control receptor and agonist (B2AR BacMam and 10mM isoproterenol in HCl). 10mL size is available as well. Other Montana Molecular tools including BacMam-packaged GPCRs, green fluorescent membrane-targeted or cytosolic cAMP sensors, or green fluorescent sensors for DAG or Calcium can easily be co-expressed with this assay.

Measure real-time cAMP signaling kinetics with this flag-tagged S15 cADDis cAMP sensor. Add fS15-tagged cADDis to your cells and measure robust signals the next day on a fluorescence imaging system. Increasing red fluorescence indicates cAMP increase. This assay is genetically encoded and packaged in BacMam for efficient transduction in most cell types - including uniform and near 100% efficiency in HEK293 cells as well as expression in many primary or iPSC-derived cells. The kit includes the cAMP sensor BacMam, 500mM sodium butyrate in H2O, and a positive control receptor and agonist (B2AR BacMam and 10mM isoproterenol in HCl). 10mL size is available as well. Other Montana Molecular tools including BacMam-packaged GPCRs, green fluorescent nucleus-targeted or cytosolic cAMP sensors, or green fluorescent sensors for DAG or PIP2 can easily be co-expressed with this assay.

Measure Calcium signaling kinetics, screen GPCRs for Gq activity, and more with this live cell fluorescent assay. Add the green fluorescent G-GECO Calcium sensor to your cells and measure robust signals the next day on an automated plate reader or fluorescence imaging system. This assay is genetically encoded and packaged in BacMam for efficient transduction in most cell types - including uniform and near 100% efficiency in HEK293 cells as well as expression in many primary or iPSC-derived cells. The kit includes the G-GECO sensor BacMam, 500mM sodium butyrate in H2O, and a positive control receptor and agonist (hM1R BacMam and 25mM carbachol in H2O). 30mL and 60mL sizes are available as well. Other Montana Molecular tools including BacMam-packaged GPCRs or ion channels can easily be co-expressed with this assay.

Measure Calcium signaling kinetics, screen GPCRs for Gq activity, and more with this live cell fluorescent assay. Add the green fluorescent G-GECO Calcium sensor to your cells and measure robust signals the next day on an automated plate reader or fluorescence imaging system. This assay is genetically encoded and packaged in BacMam for efficient transduction in most cell types - including uniform and near 100% efficiency in HEK293 cells as well as expression in many primary or iPSC-derived cells. The kit includes the G-GECO sensor BacMam, 500mM sodium butyrate in H2O, and a positive control receptor and agonist (hM1R BacMam and 25mM carbachol in H2O). 10mL and 60mL sizes are available as well. Other Montana Molecular tools including BacMam-packaged GPCRs or ion channels can easily be co-expressed with this assay.

Measure Calcium signaling kinetics, screen GPCRs for Gq activity, and more with this live cell fluorescent assay. Add the green fluorescent G-GECO Calcium sensor to your cells and measure robust signals the next day on an automated plate reader or fluorescence imaging system. This assay is genetically encoded and packaged in BacMam for efficient transduction in most cell types - including uniform and near 100% efficiency in HEK293 cells as well as expression in many primary or iPSC-derived cells. The kit includes the G-GECO sensor BacMam, 500mM sodium butyrate in H2O, and a positive control receptor and agonist (hM1R BacMam and 25mM carbachol in H2O). 10mL and 30mL sizes are available as well. Other Montana Molecular tools including BacMam-packaged GPCRs or ion channels can easily be co-expressed with this assay.

Measure Calcium signaling kinetics, screen GPCRs for Gq activity, and more with this live cell fluorescent assay. Add the red fluorescent R-GECO Calcium sensor to your cells and measure robust signals after 30 hours on an automated plate reader or fluorescence imaging system. This assay is genetically encoded and packaged in BacMam for efficient transduction in most cell types - including uniform and near 100% efficiency in HEK293 cells as well as expression in many primary or iPSC-derived cells. The kit includes the R-GECO sensor BacMam, 500mM sodium butyrate in H2O, and a positive control receptor and agonist (hM1R BacMam and 25mM carbachol in H2O). 10mL and 30mL sizes are available as well. Other Montana Molecular tools including BacMam-packaged GPCRs or ion channels can easily be co-expressed with this assay.

Sandwich TR-FRET immunoassay for quantitative detection of human Interferon-beta (hIFNβ) in cell culture supernatants. The kit does not include a recombinant human IFNβ standard, which must be purchased separately from R&D Systems® (catalogue number 8499-IF).

Sandwich TR-FRET immunoassay for quantitative detection of human Interferon-beta (hIFNβ) in cell culture supernatants. The kit does not include a recombinant human IFNβ standard, which must be purchased separately from R&D Systems® (catalogue number 8499-IF).

Sandwich TR-FRET immunoassay for quantitative detection of human Interferon-beta (hIFNβ) in cell culture supernatants. The kit does not include a recombinant human IFNβ standard, which must be purchased separately from R&D Systems® (catalogue number 8499-IF).