Cells

Please note this product may be subject to fees, we invite you to contact your local office. Recombinant CHO-K1 cells constitutively expressing both the human BCMA protein (B-Cell Maturation Antigen or CD269, GenBank accession #NM_001192) and the Gaussia Luciferase (Δ Signal peptide). Surface expression of BCMA was confirmed by flow cytometry.

Please note this product may be subject to fees, we invite you to contact your local office. Recombinant RPMI 8226 cells constitutively expressing firefly (Photinus pyralis) luciferase.

Please note this product may be subject to fees, we invite you to contact your local office. Cryopreserved vial (30 x 10^6 cells) of freshly isolated primary human peripheral blood mononuclear cells (PBMCs) from a healthy donor, isolated from leukapheresis / apheresis samples using Ficoll gradient. After isolation, the PBMCs were stained to identify sub populations and evaluated for viability by flow cytometry. Cells were cryopreserved in serum-free Cryostor CS10 at a controlled rate.

Please note this product may be subject to fees, we invite you to contact your local office. Recombinant HEK293 cell line expressing human PDE1B (phosphodiesterase 1B, accession number NM_000924). N-terminal His-tagged human PDE1B has been stably expressed in HEK293 cell line and its expression was confirmed by Western blotting. The function of PDE1B was characterized by a cell-based cGMP assay. In this assay system, cells co-express atrial natriuretic peptide (ANP) receptor A (a guanylate cyclase), cyclic nucleotide5gated cation channel CNGA2, and the photoprotein aequorin. Intracellular cGMP level is monitored via aequorin luminescence induced by Ca²⁺ influx through CNGA2, acting as the intracellular cGMP sensor.

Please note this product may be subject to fees, we invite you to contact your local office. The AP-1 Reporter - HEK293 Cell Linee is designed for monitoring the activity of the JNK signaling pathway. The AP1 Reporter - HEK293 cell line contains a firefly luciferase gene under the control of AP1-responsive elements that are stably integrated into HEK293 cells.

Please note this product may be subject to fees, we invite you to contact your local office. The SRE Reporter - HEK293 cell line is designed for monitoring the activity of the JAK/STAT signaling pathway. The SRE Reporter - HEK293 cell line contains a firefly luciferase gene under the control of SRE responsive elements stably integrated into HEK293 cells, resulting in an ERK pathway-responsive reporter cell line.

Please note this product may be subject to fees, we invite you to contact your local office. Recombinant HEK293 cell line expressing human PDE7A (phosphodiesterase 7A, accession number NM_002603). N-terminal FLAG-tagged human PDE7A has been stably expressed in a human embryonic kidney (HEK293) cell line. PDE7A expression was confirmed by Western blotting. The regulation of intracellular level of cAMP by PDE7A in PDE7A stably-expressed HEK293 cells was characterized by a cell-based reporter assay using pCRE-luc reporter vector. pCRE-luc contains a luciferase gene that is under the control of the cAMP response element (CRE). When cells transiently transfected with pCRE-luc reporter were activated by forskolin, the level of cAMP was upregulated in parental HEK293 cells, inducing the expression of the luciferase reporter, whereas hPDE7A-HEK293 cells showed reduction in the level of cAMP that resulted in lowered expression of luciferase. Inhibition of PDE7A activity by BRL 50481, a PDE7A i

Please note this product may be subject to fees, we invite you to contact your local office. Recombinant HEK293 cell line expressing rat PDE7A (phosphodiesterase 7A, accession number NM_031080). N-terminal FLAG-tagged rat PDE7A has been stably expressed in a human embryonic kidney (HEK293) cell line and its expression was confirmed by Western blotting. The regulation of intracellular level of cAMP by rat PDE7A in rat PDE7A stably-expressed HEK293 cells was characterized by a cell-based reporter assay using pCRE-luc reporter vector. pCRE-luc contains a luciferase gene that is under the control of the cAMP response element (CRE). When cells transiently transfected with pCRE-luc reporter were activated by forskolin, the level of cAMP was upregulated in parental HEK293 cells inducing the expression of luciferase reporter, whereas rat PDE7A-HEK293 cells showed reduction in the level of cAMP, resulting in lowered levels of luciferase expression. Inhibition of PDE7A activity by BRL 50481, a

Please note this product may be subject to fees, we invite you to contact your local office. The Gli Reporter - NIH3T3 Cell Line is designed for monitoring the activity of the hedgehog signaling pathway. The Gli Reporter - NIH3T3 Cell Line contains the firefly luciferase gene under the control of Gli responsive elements stably integrated into NIH3T3 cells. Luciferase expression correlates with activation of the hedgehog signaling pathway.