Cells

Please note this product may be subject to fees, we invite you to contact your local office. Recombinant HEK293 cell line expressing human PDE10A2 (phosphodiesterase 10A, accession number NM_006661). N-terminal His-tagged human PDE10A2 has been stably expressed in a human embryonic kidney (HEK293) cell line. PDE10A2 expression was confirmed by Western blotting. The function of PDE10A2 was confirmed by cAMP detection assay. Forskolin is commonly used to raise levels of intracellular cyclic AMP (cAMP). When HEK293 cells are activated by forskolin, cAMP levels are upregulated in parental cells, whereas cells overexpressing PDE10A show reduction in cAMP accumulation. Inhibition of PDE10A activity restores cAMP levels.

Please note this product may be subject to fees, we invite you to contact your local office. Recombinant HEK293 cell line expressing human PDE7B (phosphodiesterase 7B, accession number NM_018945). <br> Human PDE7B has been stably expressed in a human embryonic kidney (HEK293) cell line. PDE7B expression was confirmed by Western blotting. The function of expressed PDE7B was validated by cAMP detection assay. The regulation of intracellular level of cAMP by PDE7B in PDE7B stably-expressed HEK293cells was characterized by a cell-based reporter assay using pCRE-luc reporter vector. pCRE-luc contains the luciferase gene that is under the control of cAMP response element (CRE). Elevation of intracellular cAMP activates cAMP response element binding protein (CREB) to bind CRE and induces the expression of luciferase. When cells transiently transfected with pCRE-luc reporter were activated by forskolin, the level of cAMP was upregulated in parental HEK293 cells inducing the expression of the l

Please note this product may be subject to fees, we invite you to contact your local office. The PDE7A/CRE Reporter-HEK293 line contains the firefly luciferase gene under the control of CRE as well as a constitutive expression construct for human PDE7A (phosphodiesterase 7A, accession number NM_002603), both stably integrated into HEK293 cells. The luciferase expression level from the CRE reporter is used to monitor the activity of PDE7A in the cells.

Please note this product may be subject to fees, we invite you to contact your local office. Human IL-2 reporter construct is stably integrated into the genome of Jurkat T-cells. The firefly luciferase gene is controlled by a human IL-2 promoter.

Please note this product may be subject to fees, we invite you to contact your local office. The Wnt Signaling Pathway TCF/LEF Reporter (Luc) - HEK293 Cell Line is designed for monitoring the activity of the Wnt/b-catenin signaling pathway. The Wnt Signaling Pathway TCF/LEF Reporter (Luc) - HEK293 Cell Line contains a firefly luciferase gene under the control of TCF/LEF responsive elements stably integrated into HEK293 cells. This cell line is validated for the response to the stimulation of mouse Wnt3a and to the treatment with an inhibitor of Wnt/b-catenin signaling pathway.

Please note this product may be subject to fees, we invite you to contact your local office. The RARα Reporter (Luc)-HEK293 Cell Line contains a firefly luciferase gene under the control of retinoic acid response elements stably integrated into HEK293 cells along with full length human RARα (NM_000964).

Please note this product may be subject to fees, we invite you to contact your local office. The CFTR HEK293 Cell Line expresses full length, wild-type human cystic fibrosis transmembrane conductance regulator (CFTR) protein (Genbank #P13569, NP_000483.3), with a C-terminal Streptavidin-Binding Peptide (SBP) tag. Expression must be induced ≥24 hours prior to an experiment using 1 µg/ml Doxycycline and 3 mM Na-butyrate. The inducible expression of CFTR was confirmed by Western blotting and flow cytometry.

Please note this product may be subject to fees, we invite you to contact your local office. Stable recombinant HEK293 cell line expressing human Nav1.7 (Genbank # Q15858) fused at the C-terminus to Green Fluorescent Protein [Ex. ~395 nm, 475 nm; em ~510 nm] and with Cterminal Streptavidin-Binding Peptide (SBP) fusion. Nav1.7 is a tetrodotoxin-sensitive voltagegated sodium channel type IX subunit alpha (SCN9A).

Please note this product may be subject to fees, we invite you to contact your local office. The ISRE Reporter - HEK293 Cell Line is designed for monitoring the activity of the JAK/STAT signaling pathway. The ISRE Reporter - HEK293 Cell Line contains the firefly luciferase gene under the control of ISRE stably integrated into HEK293 cells.