Labelling

Halo-Flipper is a fluorescent probe that specifically labels Halo-tag™* and reports membrane tension changes through its fluorescence lifetime changes. It contains a Chloroalcane Halo-tag™* ligand as well as a tethered Flipper fluorophore which senses changes of the organization of lipid bilayer membranes surrounding the Halo-tag™* protein . Halo-Flipper spontaneously labels Halotag expressing cells and is only fluorescent when inserted in a lipid membrane. It has a broad absorption and emission spectrum, excitation can be commonly performed with a 488nm laser, while emission is collected between 575 and 625nm. It is the perfect tool to precisely localize the Flipper-TR membrane tension fluorophore within cells. ChloroAlkane (CA) is the substrate of the self labeling tag Halo-tag™*. Upon reaction with a CA derivative, Halo-tag™* forms a covalent bond with the substrate. It allows to permanently attach a fluorescent label to any protein of interest (POI) expressed as Halo-tag™* fusion.

SPY505-CA is the ChloroAlkane (CA) derivative of our SPY505 fluorophore. It emits light in the green part of the UV-ViS spectrum, it is fluorogenic, highly cell permeable and well suited for STED and SIM superresolution imaging. SPY505-CA can be imaged with a standard FITC or YFP filterset. It can be used for widefield, confocal, SIM or STED imaging in living or fixed cells and tissue. It has the same structure as MaP510-halo, the name used in its original publication in Nature Chemistry. ChloroAlkane (CA) is the substrate of the self labeling tag Halo-tag™*. Upon reaction with a CA derivative, Halo-tag™* forms a covalent bond with the substrate. It allows to permanently attach a fluorescent label to any protein of interest (POI) expressed as Halo-tag™* fusion. Contains 1 vial of SPY505-CA (lyophilized). *Halo-tag™ is a registered trademark of Promega Corporation.

SPY555-CA is the ChloroAlkane (CA) derivative of our SPY555 fluorophore. It emits light in the orange part of the UV-ViS spectrum, it is fluorogenic, highly cell permeable and well suited for STED and SIM superresolution imaging. SPY555-CA can be imaged with a standard Cy3 filterset. It can be used for widefield, confocal, SIM or STED imaging in living or fixed cells and tissue. It has the same structure as MaP555-halo or MaP555-CA, the name used in its original publication in Nature Chemistry. ChloroAlkane (CA) is the substrate of the self labeling tag Halo-tag™*. Upon reaction with a CA derivative, Halo-tag™* forms a covalent bond with the substrate. It allows to permanently attach a fluorescent label to any protein of interest (POI) expressed as Halo-tag™* fusion. Contains 1 vial of SPY555-CA (lyophilized) *Halo-tag™ is a registered trademark of Promega Corporation.

SPY555-golgi is a bright, Orange & non toxic live cell golgi probe based on our SPY™ dyes series. Its optimized structure allows quick labeling of the golgi apparatus in live cells with high specificity and low background. SPY555-golgi stains the golgi apparatus in live cells without the need for genetic manipulation or overexpression of fluorescent proteins. Its absorbance and emission spectra are similar to TMR. SPY555-golgi enables multicolor imaging with SPY505, SPY595, SPY650, SPY700, SiR and GFP. SPY555-golgi can be imaged with standard TMR or Cy3 filtersets. It can be used for confocal, SIM or STED imaging in living cells and tissue. Contains 1 vial of SPY555-golgi (lyophilized).

CA_TestKit combines our 3 Halo-tag™ subtrates SPY505-CA, SPY555-CA and SiR-CA, offering a wide flexibility for Halo-tag™ fusion proteins labeling in live and fixed cells. The ligands are all bright, highly fluorogenic, and cell permeable. The 3 fluorophores are covering 3 distinct and very common imaging channels: FITC, TMR/Cy3 and Cy5. Kit contains 3 separate tubes containing 3 nmol of each Halo-tag™ substrate (ca. 30 stainings/tube). Halo-tag™ is a registered trademark of Promega Corporation.

SiR-CA is the ChloroAlkane (CA) derivative of our SiR fluorophore. It emits light in the far red part of the UV-ViS spectrum, it is fluorogenic, highly cell permeable and well suited for STED and SIM superresolution imaging. SiR-CA can be imaged with a standard Cy5 filterset. It can be used for widefield, confocal, SIM or STED imaging in living or fixed cells and tissue. It has the same structure as SiR-Halo, the name used in its original publication in Nature Chemistry. ChloroAlkane (CA) is the substrate of the self labeling tag Halo-tag™*. Upon reaction with a CA derivative, Halo-tag™* forms a covalent bond with the substrate. It allows to permanently attach a fluorescent label to any protein of interest (POI) expressed as Halo-tag™* fusion. Contains 1 vial of SiR-CA (lyophilized). *Halo-tag™ is a registered trademark of Promega Corporation.

SPY650-golgi is a bright, far red & non toxic live cell golgi probe based on our SPY™ dyes series. Its optimized structure allows quick labeling of the golgi apparatus in live cells with high specificity and low background. SPY650-golgi stains the golgi apparatus in live cells without the need for genetic manipulation or overexpression of fluorescent proteins. Its absorbance and emission spectra are similar to Cy5. SPY650-golgi enables multicolor imaging with SPY505, SPY555, SPY595, SPY700, GFP or m-cherry. SPY650-golgi can be imaged with standard Cy5 filtersets. It can be used for confocal, SIM or STED imaging in living cells and tissue. Contains 1 vial of SPY650-golgi (lyophilized).

SPY700-CA is the ChloroAlkane (CA) derivative of our SPY700 fluorophore. It emits light in the far red part of the UV-ViS spectrum, it is fluorogenic, highly cell permeable. SPY700-CA can be imaged with Cy5 filtersets but using a 700 nm LP emission filter is best. It can be used for widefield, confocal or SIM imaging in living or fixed cells and tissue. It has the same structure as MaP700-Halo, the name used in its original publication in Nature Chemistry. ChloroAlkane (CA) is the substrate of the self labeling tag Halo-tag™*. Upon reaction with a CA derivative, Halo-tag™* forms a covalent bond with the substrate. It allows to permanently attach a fluorescent label to any protein of interest (POI) expressed as Halo-tag™* fusion. Contains 1 vial of SPY700-CA (lyophilized). *Halo-tag™ is a registered trademark of Promega Corporation.

CPY-CA is the ChloroAlkane (CA) derivative of the carbopyronine (CPY) fluorophore. It emits light in the red part of the UV-ViS spectrum, it is fluorogenic, highly cell permeable and well suited for STED and SIM superresolution imaging. CPY-CA enables multicolor imaging with SPY505, SPY555, SPY650, SPY700, SiR and GFP. CPY-CA can be imaged with e.g. using 610/20 nm and 650/40 nm excitation and emission filters respectively. ChloroAlkane (CA) is the substrate of the self labeling tag Halo-tag™*. Upon reaction with a CA derivative, Halo-tag™* forms a covalent bond with the substrate. It allows to permanently attach a fluorescent label to any protein of interest (POI) expressed as Halo-tag™* fusion. Contains 1 vial of CPY-CA (lyophilized). *Halo-tag™ is a registered trademark of Promega Corporation.