Labelling

BP Fluor 800 Picolyl Azide is a conceptually new class of sterically shielded NIR cyanine heptamethine dye with emission wavelengths >740 nm. This dye contains two shielding PEG arms directly over both faces of the heptamethine fluorochrome blocking any undesired bimolecular association processes, resulting in a significant increase in fluorescence brightness. BP Fluor 800 Picolyl Azide reacts with the alkyne group in antibody, proteins, peptides, amino-modified oligos, and other target molecules. It is specially to be used to detect low abundance targets. Compared with conventional azides, picolyl azide will allow to use at least 10 fold less copper catalyst to yield the same click reaction rate as regular azide one, because picolyl moiety can chelate Cu to raise the effective concentration of Cu(I) at the reaction site.

ATTO488-Charybdotoxin is a fluorescent version of Charybdotoxin. Charybdotoxin (ChTx) is a 37 amino acid peptide isolated from the venom of the scorpion Leiurus quinquestriatus hebraeus that blocks voltage-gated and large conductance Ca2+ activated K+ channels KCa1.1 in nanomolar concentrations (IC50~ 3 nM). This blockade causes hyperexcitability of the nervous system. The toxin reversibly blocks channel activity by interacting at the external pore of the channel protein with an apparent Kd of 2.1 nM. ChTX also blocks KCa3.1 (IC50 5 nM), Kv1.2 (IC50 14 nM), Kv1.3 (IC50 2.6 nM) and Kv1.6 (IC50 2 nM) channels. ATTO488: λex 500nm , λem 520nm