Labelling

CA_TestKit combines our 3 Halo-tag™ subtrates SPY505-CA, SPY555-CA and SiR-CA, offering a wide flexibility for Halo-tag™ fusion proteins labeling in live and fixed cells. The ligands are all bright, highly fluorogenic, and cell permeable. The 3 fluorophores are covering 3 distinct and very common imaging channels: FITC, TMR/Cy3 and Cy5. Kit contains 3 separate tubes containing 3 nmol of each Halo-tag™ substrate (ca. 30 stainings/tube). Halo-tag™ is a registered trademark of Promega Corporation.

SiR-CA is the ChloroAlkane (CA) derivative of our SiR fluorophore. It emits light in the far red part of the UV-ViS spectrum, it is fluorogenic, highly cell permeable and well suited for STED and SIM superresolution imaging. SiR-CA can be imaged with a standard Cy5 filterset. It can be used for widefield, confocal, SIM or STED imaging in living or fixed cells and tissue. It has the same structure as SiR-Halo, the name used in its original publication in Nature Chemistry. ChloroAlkane (CA) is the substrate of the self labeling tag Halo-tag™*. Upon reaction with a CA derivative, Halo-tag™* forms a covalent bond with the substrate. It allows to permanently attach a fluorescent label to any protein of interest (POI) expressed as Halo-tag™* fusion. Contains 1 vial of SiR-CA (lyophilized). *Halo-tag™ is a registered trademark of Promega Corporation.

SPY650-golgi is a bright, far red & non toxic live cell golgi probe based on our SPY™ dyes series. Its optimized structure allows quick labeling of the golgi apparatus in live cells with high specificity and low background. SPY650-golgi stains the golgi apparatus in live cells without the need for genetic manipulation or overexpression of fluorescent proteins. Its absorbance and emission spectra are similar to Cy5. SPY650-golgi enables multicolor imaging with SPY505, SPY555, SPY595, SPY700, GFP or m-cherry. SPY650-golgi can be imaged with standard Cy5 filtersets. It can be used for confocal, SIM or STED imaging in living cells and tissue. Contains 1 vial of SPY650-golgi (lyophilized).

SPY700-CA is the ChloroAlkane (CA) derivative of our SPY700 fluorophore. It emits light in the far red part of the UV-ViS spectrum, it is fluorogenic, highly cell permeable. SPY700-CA can be imaged with Cy5 filtersets but using a 700 nm LP emission filter is best. It can be used for widefield, confocal or SIM imaging in living or fixed cells and tissue. It has the same structure as MaP700-Halo, the name used in its original publication in Nature Chemistry. ChloroAlkane (CA) is the substrate of the self labeling tag Halo-tag™*. Upon reaction with a CA derivative, Halo-tag™* forms a covalent bond with the substrate. It allows to permanently attach a fluorescent label to any protein of interest (POI) expressed as Halo-tag™* fusion. Contains 1 vial of SPY700-CA (lyophilized). *Halo-tag™ is a registered trademark of Promega Corporation.

CPY-CA is the ChloroAlkane (CA) derivative of the carbopyronine (CPY) fluorophore. It emits light in the red part of the UV-ViS spectrum, it is fluorogenic, highly cell permeable and well suited for STED and SIM superresolution imaging. CPY-CA enables multicolor imaging with SPY505, SPY555, SPY650, SPY700, SiR and GFP. CPY-CA can be imaged with e.g. using 610/20 nm and 650/40 nm excitation and emission filters respectively. ChloroAlkane (CA) is the substrate of the self labeling tag Halo-tag™*. Upon reaction with a CA derivative, Halo-tag™* forms a covalent bond with the substrate. It allows to permanently attach a fluorescent label to any protein of interest (POI) expressed as Halo-tag™* fusion. Contains 1 vial of CPY-CA (lyophilized). *Halo-tag™ is a registered trademark of Promega Corporation.

BP Fluor 800 Picolyl Azide is a conceptually new class of sterically shielded NIR cyanine heptamethine dye with emission wavelengths >740 nm. This dye contains two shielding PEG arms directly over both faces of the heptamethine fluorochrome blocking any undesired bimolecular association processes, resulting in a significant increase in fluorescence brightness. BP Fluor 800 Picolyl Azide reacts with the alkyne group in antibody, proteins, peptides, amino-modified oligos, and other target molecules. It is specially to be used to detect low abundance targets. Compared with conventional azides, picolyl azide will allow to use at least 10 fold less copper catalyst to yield the same click reaction rate as regular azide one, because picolyl moiety can chelate Cu to raise the effective concentration of Cu(I) at the reaction site. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

ATTO488-Charybdotoxin is a fluorescent version of Charybdotoxin. Charybdotoxin (ChTx) is a 37 amino acid peptide isolated from the venom of the scorpion Leiurus quinquestriatus hebraeus that blocks voltage-gated and large conductance Ca2+ activated K+ channels KCa1.1 in nanomolar concentrations (IC50~ 3 nM). This blockade causes hyperexcitability of the nervous system. The toxin reversibly blocks channel activity by interacting at the external pore of the channel protein with an apparent Kd of 2.1 nM. ChTX also blocks KCa3.1 (IC50 5 nM), Kv1.2 (IC50 14 nM), Kv1.3 (IC50 2.6 nM) and Kv1.6 (IC50 2 nM) channels. ATTO488: λex 500nm , λem 520nm

Amino-PEG46-Alcohol represents a bifunctional PEG linker with a terminal amino group that reacts with carboxylic acids, activated NHS esters, carbonyls (ketone, aldehyde) etc., and hydroxyl which reacts with a variety of functional groups. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.