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29 cases of esophagus squamous cell carcinoma(21 cases tumor with matched NAT, 1 core/case; 8 cases tumor tissue 1 core/case):squamous cell carcinomas,basal cell squamous carcinoma, spindle cell squamous carcinoma,adenocarcinoma, adenosquamous carconoma,undifferentiated carcinoma. Diameter: 2.0 mm. Cores: 50. Cases: 29.