Buffers & Concentrates

This blocking buffer is specifically designed for Western blotting using fluorochrome conjugated antibodies. Pure nitrocellulose membrane is recommended for maximum performance. Other membranes, such as PVDF or nitrocellulose embedded in a support can be used, but may generate elevated backgrounds. Protein should be transferred from gel to membrane using standard protocols. Blocking buffer can be used for membrane blocking and to dilute both primary and secondary antibodies. Blocking Buffer for Fluorescent Western Blotting is suitable for use with imaging systems produced by Bio-Rad Laboratories, GE Healthcare, Alpha Innotech, FujiFilm Life Science, Licor Biosciences, UVP and Syngene.

BPS Bacterial Protein extraction Buffer enables extraction of both soluble and insoluble (inclusion bodies) proteins from bacteria. The buffer provides high efficiency recovery of over-expressed proteins and is fully compatible with HIS/GST-tag purification procedures and standard protein concentration determination assays. The buffer can also be used for protein extraction from insect cells.

BPS Bacterial Protein extraction Buffer enables extraction of both soluble and insoluble (inclusion bodies) proteins from bacteria. The buffer provides high efficiency recovery of over-expressed proteins and is fully compatible with HIS/GST-tag purification procedures and standard protein concentration determination assays. The buffer can also be used for protein extraction from insect cells.

The 3x BRD Homogeneous Assay Buffer 1 is optimized for use with the following BPS Bioscience assay kits: <br />• BRD2 (BD2) Inhibitor Screening Assay Kit #32522 384 reactions <br />• BRD3 (BD1) Inhibitor Screening Assay Kit #32513 384 reactions <br />• BRD3 (BD2) Inhibitor Screening Assay Kit #32523 384 reactions <br />• BRD4 (BD1) Inhibitor Screening Assay Kit #32514 384 reactions <br />• BRD4 (BD2) Inhibitor Screening Assay Kit #32524 384 reactions NOTE: This product was formerly known as 3x BRD Assay Buffer.

The 3x BRD Detection Buffer 1 is optimized for use with the following BPS Bioscience assay kits: • BRD2 (BD2) Inhibitor Screening Assay Kit #32522 384 reactions <br />• BRD3 (BD2) Inhibitor Screening Assay Kit #32523 384 reactions <br />• BRD3 (BD1) Inhibitor Screening Assay Kit #32513 384 reactions <br />• BRD4 (BD1) Inhibitor Screening Assay Kit #32514 384 reactions <br />• BRD4 (BD2) Inhibitor Screening Assay Kit #32524 384 reactions NOTE: This product was formerly known as 3x BRD Detection Buffer.

The 3x BRD Homogeneous Detection Buffer 2 is optimized for use with the following BPS Bioscience assay kits: <br />• ATAD2A Inhibitor Screening Assay Kit #32601 384 reactions <br />• ATAD2B Inhibitor Screening Assay Kit #32605 384 reactions <br />• BRD1 Inhibitor Screening Assay Kit #32521 384 reactions <br />• BRD4 (BD2) Inhibitor Screening Assay Kit #32524 384 reactions <br />• BRD9 (BD1) Inhibitor Screening Assay Kit #32519 384 reactions <br />• BRPF3 Inhibitor Screening Assay Kit #32608 384 reactions <br />• CECR2 Inhibitor Screening Assay Kit #32611 384 reactions <br />• CREBBP Inhibitor Screening Assay Kit #32607 384 reactions <br />• SMARCA2 Inhibitor Screening Assay Kit #32610 384 reactions <br />• SMARCA4/BRG1 Inhibitor Screening Assay Kit #32609 384 reactions <br />• TAF1 (BD1/BD2) Inhibitor Screening Assay Kit #32604 384 reactions <br />• TAF1L (BD2) Inhibitor Screening Assay Kit #32602 384 reactions <br />• TAF1L (BD1/BD2) Inhibitor Screening Assay Kit #32603

The 3x BRD Homogeneous Assay Buffer 2 is optimized for use with the following BPS Bioscience assay kits: <br />• ATAD2A Inhibitor Screening Assay Kit #32601 384 reactions <br />• ATAD2B Inhibitor Screening Assay Kit #32605 384 reactions <br />• BRD1 Inhibitor Screening Assay Kit #32521 384 reactions <br />• BRD4 (BD2) Inhibitor Screening Assay Kit #32524 384 reactions <br />• BRD9 (BD1) Inhibitor Screening Assay Kit #32519 384 reactions <br />• BRPF3 Inhibitor Screening Assay Kit #32608 384 reactions <br />• CECR2 Inhibitor Screening Assay Kit #32611 384 reactions <br />• CREBBP Inhibitor Screening Assay Kit #32607 384 reactions <br />• SMARCA2 Inhibitor Screening Assay Kit #32610 384 reactions <br />• SMARCA4/BRG1 Inhibitor Screening Assay Kit #32609 384 reactions <br />• TAF1 (BD1/BD2) Inhibitor Screening Assay Kit #32604 384 reactions <br />• TAF1L (BD2) Inhibitor Screening Assay Kit #32602 384 reactions <br />• TAF1L (BD1/BD2) Inhibitor Screening Assay Kit #32603 384 r

The 3x BRD TR-FRET Assay Buffer 1 is optimized for use with the following BPS Bioscience assay kits: ATAD2A TR-FRET Assay Kit #32618 384 reactions ATAD2B TR-FRET Assay Kit #32620 384 reactions BPTF/FALZ TR-FRET Assay Kit #32632 384 reactions BRD1 TR-FRET Assay Kit #32625 384 reactions BRD2 (BD1+BD2) TR-FRET Assay Kit #32614 384 reactions BRD3 (BD1+BD2) TR-FRET Assay Kit #32615 384 reactions BRD4 (BD1+BD2) TR-FRET Assay Kit #32612 384 reactions BRD4 (BD1) TR-FRET Assay Kit #32613 384 reactions BRD4 (BD2) TR-FRET Assay Kit #32617 384 reactions BRD9 TR-FRET Assay Kit #32621 384 reactions BRD9 TR-FRET Assay Kit #32621 384 reactions BRPF3 TR-FRET Assay Kit #32626 384 reactions CECR2 TR-FRET Assay Kit #32622 384 reactions CREBBP TR-FRET Assay Kit #32619 384 reactions TAF1 (BD2) TR-FRET Assay Kit #32627 384 reactions TAF1 (BD1+BD2) TR-FRET Assay Kit #32628 384 reactions TAF1L (BD1+BD2) TR-FRET Assay Kit #32629 384 reactions WDR9/BRWD1 TR-FRET Assay Kit #32631 384 reactions

The 3x BRD TR-FRET Assay Buffer 2 is optimized for use with the following BPS Bioscience assay kits: • TRIM24 TR-FRET Assay Kit #32630 384 reactions