DNA & Clones

The pE-SUMOpro3 vector is for the expression of recombinant SUMO3 fusion proteins (containing an N-terminal His6 tag) in <em>E. coli</em>. Production of your protein of interest (POI) is driven through the powerful T7 RNA polymerase-promoter system, with ampicillin resistance facilitating plasmid selection and stability.

The pE-SUMOstar vector is for the expression of recombinant SUMOstar fusion proteins (containing an N-terminal His6 tag) in <em>E. coli</em>. Production of your protein of interest (POI) is driven through the powerful T7 RNA polymerase-promoter system, with kanamycin resistance facilitating plasmid selection and stability.

The pE-SUMOstar vector is for the expression of recombinant SUMOstar fusion proteins (containing an N-terminal His₆ tag) in <em>E. coli</em>. Production of your protein of interest (POI) is driven through the powerful T7 RNA polymerase-promoter system, with ampicillin resistance facilitating plasmid selection and stability.

pI-SUMOstar is used for intracellular expression of SUMOstar fusion proteins (containing an N-terminal His6 tag) in baculovirus expression systems (BEVS). Bacmid generation from this plasmid requires DH10bac for transposition into the baculoviral genome. Expression of your protein of interest (POI) is driven by the late stage viral polyhedrin promoter (<em>polh</em>). pI-SUMOstar contains an ampicillin marker for propagation in <em>E. coli</em>.

pI-secSUMOstar is used for expression and secretion of SUMOstar fusion proteins (containing an N-terminal His6 tag) in baculovirus expression vector systems (BEVS). Bacmid generation from this plasmid requires the use of DH10bac for transposition into the baculoviral genome. Expression of fusion proteins is driven by the late stage viral polyhedrin promoter (<em>polh</em>). Your protein of interest (POI) is targeted for processing and secretion through fusion with the secretion signal sequence from the envelope surface glycoprotein 67 (gp67) of <em>Autographa californica</em>.  pI-secSUMOstar contains an ampicillin marker for propagation in <em>E. coli</em>.

The pM-SUMOstar vector allows for cytosolic expression of SUMO fusion constructs in a mammalian cell system.  Expression is driven by the strong constitutive human cytomegalovirus (CMV) promoter and efficiently terminated by the bovine growth hormone (BGH) polyadenylation sequence.  SUMO fusions can be expressed transiently or as stable chromosomal integrants in a wide variety of cell lines.  Expression can be further enhanced by episomal replication in SV40 infected cell lines or those that express the SV40 large T antigen (i.e. HEK-293T).

The pM-secSUMOstar vector allows for expression and secretion of SUMO fusion constructs in a mammalian cell system.  Expression is driven by the strong constitutive human cytomegalovirus (CMV) promoter and efficiently terminated by the bovine growth hormone (BGH) polyadenylation sequence.  Proteins are directed to the secretory pathway by the mouse IgG kappa secretory signal, which precedes a His6 tag. SUMOstar fusions can be expressed transiently or as stable chromosomal integrants in a wide variety of cell lines.  Expression can be further enhanced by episomal replication in SV40 infected cell lines or those that express the SV40 large T antigen (i.e. HEK-293T).

The Ub-CHOP2-Reporter Deubiquitylation Assay consists of ubiquitin fused to a reporter enzyme, as well as a separate reagent substrate for the reporter enzyme. When fused to ubiquitin, the reporter is rendered catalytically inactive. Following cleavage of the Ub-reporter system by the isopeptidase, the free (and now active) reporter subsequently acts upon its substrate. Thus, in this coupled assay, the signal generated by cleavage of the reporter enzyme’s substrate is a quantitative measure of isopeptidase activity.-\n<h3><strong>Contents</strong></h3>-\n-- Ub-CHOP2-Reporter System: 1 x 375 &#0956;L (4 &#0956;M)-\n-- USP2 (Control Isopeptidase): 2 x 10 &#0956;L (2 &#0956;M)-\n-- Reporter Substrate 2: 1 x 30 &#0956;L (25 &#0956;M)-\n-- Control Fluorophore 2: 1 x 20 &#0956;L (1 mM)

The NEDD8-CHOP-Reporter DeNEDDylation Assay Kit consists of NEDD8 fused to a reporter enzyme and the reporter enzyme substrate. When fused to NEDD8, the reporter is rendered catalytically inactive. Following cleavage of the NEDD8-reporter system by isopeptidase activity, the free (and now active) reporter enzyme subsequently acts upon its substrate. Thus, in this coupled assay, the signal generated by cleavage of the reporter enzyme’s substrate is a quantitative measure of isopeptidase activity.-\n<h3><strong>Contents</strong></h3>-\n-- NEDD8-CHOP2-Reporter (Reporter System): 1 x 375 &#0956;L (4 &#0956;M)-\n-- Den1 (Control Isopeptidase): 2 x 10 &#0956;L (2 &#0956;M)-\n-- Reporter Substrate 2: 1 x 30 &#0956;L (25 &#0956;M)-\n-- Control Fluorophore 2: 1 x 20 &#0956;L (1 mM)