RNA

Total RNA was prepared from cell line WM3000. WM3000 is a metastatic human melanoma cell line that displays a melanocytic morphology. This cell line contains a Q61R mutation at position 61 in the N-RAS gene. The Q61R is the most common NRAS mutation found in melanoma that is thought to occur due to UV and radiation exposure. This mutation leads to production of a constitutively active N-RAS protein that directs cells to grow and divide constantly. WM3000 cells are from same patient as WM 2032. These cells are expected to produce xenograft tumors when injected into immunocompromised mice.

Total RNA was prepared from cell line WM3000. WM3000 is a metastatic human melanoma cell line that displays a melanocytic morphology. This cell line contains a Q61R mutation at position 61 in the N-RAS gene. The Q61R is the most common NRAS mutation found in melanoma that is thought to occur due to UV and radiation exposure. This mutation leads to production of a constitutively active N-RAS protein that directs cells to grow and divide constantly. WM3000 cells are from same patient as WM 2032. These cells are expected to produce xenograft tumors when injected into immunocompromised mice.

Total RNA was prepared from cell line WM3060. WM3060 is a metastatic human melanoma cell line established from a metastatic site (left axillary nodes) in a 55-year-old male with level III malignant melanoma. The subject displayed VGP, and RGP with a tumor thickness of 3.0 mm. This cell line was derived from the same patient as the cell lines WM2013, and WM1960. This cell line contains a Q61K mutation at position 61 in the N-RAS gene which causes increased signaling via the extracellular signal-regulated MAPK/ERK kinase pathways to enhance proliferation. This cell line is wild type for BRAF, PTEN, c-KIT, and CDK4. WM3060 cells produce xenograft tumors when injected into immunocompromised mice.

Total RNA was prepared from cell line WM3060. WM3060 is a metastatic human melanoma cell line established from a metastatic site (left axillary nodes) in a 55-year-old male with level III malignant melanoma. The subject displayed VGP, and RGP with a tumor thickness of 3.0 mm. This cell line was derived from the same patient as the cell lines WM2013, and WM1960. This cell line contains a Q61K mutation at position 61 in the N-RAS gene which causes increased signaling via the extracellular signal-regulated MAPK/ERK kinase pathways to enhance proliferation. This cell line is wild type for BRAF, PTEN, c-KIT, and CDK4. WM3060 cells produce xenograft tumors when injected into immunocompromised mice.

Total RNA was prepared from cell line WM3060. WM3060 is a metastatic human melanoma cell line established from a metastatic site (left axillary nodes) in a 55-year-old male with level III malignant melanoma. The subject displayed VGP, and RGP with a tumor thickness of 3.0 mm. This cell line was derived from the same patient as the cell lines WM2013, and WM1960. This cell line contains a Q61K mutation at position 61 in the N-RAS gene which causes increased signaling via the extracellular signal-regulated MAPK/ERK kinase pathways to enhance proliferation. This cell line is wild type for BRAF, PTEN, c-KIT, and CDK4. WM3060 cells produce xenograft tumors when injected into immunocompromised mice.

Total RNA was prepared from cell line WM3061. WM3061 is a metastatic human melanoma cell line. These are small spherical cells that float easily after splitting and require 5% medium to adhere to culture flask. WM3061 cells produce xenograft tumors when injected into immunocompromised mice. This cell line contains a G13R mutation at position 13 in the N-RAS gene. Mutations affecting codon 13 lead to constitutive activation of RAS GTPase and ultimately neoplastic growth. The G13R is a rare NRAS mutation found in melanoma.

Total RNA was prepared from cell line WM3061. WM3061 is a metastatic human melanoma cell line. These are small spherical cells that float easily after splitting and require 5% medium to adhere to culture flask. WM3061 cells produce xenograft tumors when injected into immunocompromised mice. This cell line contains a G13R mutation at position 13 in the N-RAS gene. Mutations affecting codon 13 lead to constitutive activation of RAS GTPase and ultimately neoplastic growth. The G13R is a rare NRAS mutation found in melanoma.

Total RNA was prepared from cell line WM3061. WM3061 is a metastatic human melanoma cell line. These are small spherical cells that float easily after splitting and require 5% medium to adhere to culture flask. WM3061 cells produce xenograft tumors when injected into immunocompromised mice. This cell line contains a G13R mutation at position 13 in the N-RAS gene. Mutations affecting codon 13 lead to constitutive activation of RAS GTPase and ultimately neoplastic growth. The G13R is a rare NRAS mutation found in melanoma.

Total RNA was prepared from cell line WM3211. WM3211 is a tumorigenic (VGP) primary melanoma cell line with competence for metastasis. These cells display fibroblastic morphology in culture. This cell line was established from a metastatic site (lymph node) in a 34-year-old female. This cell line contains a L576P mutation at position 576 in the c-KIT gene. The L576P mutation results in an amino acid substitution at position 576 in KIT, from a Leucine (L) to a proline (P). This mutation occurs within the juxtamembrane domain. Mutant KIT proteins have increased kinase activity and transforming activity in vitro. This cell line is wild type for BRAF, PTEN, N-RAS, and CDK4. WM3211 cells produce xenograft tumors when injected into immunocompromised mice.