RNA

Total RNA was prepared from cell line WM983A. WM983A is a VGP human malignant metastatic melanoma cell line that features the V600E (Val600Glu) mutation at codon 600 in the BRAF gene. This mutation causes constitutively active kinase activity and activation of MEK and ERK signaling pathway. This cell line is wild type for PTEN, N-RAS, c-KIT, and CDK4. This cell line was derived from the same patient as the cell lines WM983B, WM983B BR and WM983C.WM983A cells produce xenograft tumors when injected into immunocompromised mice.

Total RNA was prepared from cell line WM983A. WM983A is a VGP human malignant metastatic melanoma cell line that features the V600E (Val600Glu) mutation at codon 600 in the BRAF gene. This mutation causes constitutively active kinase activity and activation of MEK and ERK signaling pathway. This cell line is wild type for PTEN, N-RAS, c-KIT, and CDK4. This cell line was derived from the same patient as the cell lines WM983B, WM983B BR and WM983C.WM983A cells produce xenograft tumors when injected into immunocompromised mice.

Total RNA was prepared from cell line WM983B. WM983B is a human metastatic melanoma cell line that was established from an inguinal node metastatic site in a 54-year-old male patient. The subject displayed malignant melanoma of polypoid type, level IV with a tumor thickness of 2.5 cm. MW983B line contains the BRAF V600E mutation that causes increased signaling via the extracellular signal-regulated MAPK/ERK kinase pathways to enhance proliferation. WM983B is wild type for PTEN, N-ras, c-KIT, and CDK4. This cell line was derived from the same patient as the cell lines WM983A, WM983B BR, and WM983C. WM983B cells produce xenograft tumors when injected into immunocompromised mice.

Total RNA was prepared from cell line WM983B. WM983B is a human metastatic melanoma cell line that was established from an inguinal node metastatic site in a 54-year-old male patient. The subject displayed malignant melanoma of polypoid type, level IV with a tumor thickness of 2.5 cm. MW983B line contains the BRAF V600E mutation that causes increased signaling via the extracellular signal-regulated MAPK/ERK kinase pathways to enhance proliferation. WM983B is wild type for PTEN, N-ras, c-KIT, and CDK4. This cell line was derived from the same patient as the cell lines WM983A, WM983B BR, and WM983C. WM983B cells produce xenograft tumors when injected into immunocompromised mice.

Total RNA was prepared from cell line WM983B. WM983B is a human metastatic melanoma cell line that was established from an inguinal node metastatic site in a 54-year-old male patient. The subject displayed malignant melanoma of polypoid type, level IV with a tumor thickness of 2.5 cm. MW983B line contains the BRAF V600E mutation that causes increased signaling via the extracellular signal-regulated MAPK/ERK kinase pathways to enhance proliferation. WM983B is wild type for PTEN, N-ras, c-KIT, and CDK4. This cell line was derived from the same patient as the cell lines WM983A, WM983B BR, and WM983C. WM983B cells produce xenograft tumors when injected into immunocompromised mice.

Total RNA was prepared from cell line WM983BBR. WM983BBR is a human metastatic melanoma cell line that displays a mesenchymal morphology. The WM983BBR cell line contains the BRAF V600E mutation that causes increased signaling via the extracellular signal-regulated MAPK/ERK kinase pathways to enhance proliferation.  WM983BBR is wild type for PTEN, N-ras, c-KIT, and CDK4. This cell line was derived from the same patient as the cell lines WM983A, WM983B, and WM983C. The WM983BBR cells produce xenograft tumors when injected into immunocompromised mice.

Total RNA was prepared from cell line WM983BBR. WM983BBR is a human metastatic melanoma cell line that displays a mesenchymal morphology. The WM983BBR cell line contains the BRAF V600E mutation that causes increased signaling via the extracellular signal-regulated MAPK/ERK kinase pathways to enhance proliferation.  WM983BBR is wild type for PTEN, N-ras, c-KIT, and CDK4. This cell line was derived from the same patient as the cell lines WM983A, WM983B, and WM983C. The WM983BBR cells produce xenograft tumors when injected into immunocompromised mice.

Total RNA was prepared from cell line WM983BBR. WM983BBR is a human metastatic melanoma cell line that displays a mesenchymal morphology. The WM983BBR cell line contains the BRAF V600E mutation that causes increased signaling via the extracellular signal-regulated MAPK/ERK kinase pathways to enhance proliferation.  WM983BBR is wild type for PTEN, N-ras, c-KIT, and CDK4. This cell line was derived from the same patient as the cell lines WM983A, WM983B, and WM983C. The WM983BBR cells produce xenograft tumors when injected into immunocompromised mice.

Total RNA was prepared from cell line WM983C. WM983C is a metastatic human melanoma cell line. This cell line features the specific V600E (Val600Glu) mutation at codon 600 in the BRAF gene. This mutation causes constitutively active kinase activity and activation of MEK and ERK signaling pathway. This cell line expresses wild type PTEN, N-RAS, c-KIT, and CDK4 genes. WM983 cells produce xenograft tumors when injected into immunocompromised mice.