Enzymes

Recombinant human CBLB (Cbl proto-oncogene B), encompassing amino acids 39-426. This construct contains an N-terminal GST-tag followed by a PreScission Protease Cleavage site to facilitate removal of the GST portion and contains the mutation of interest (Y363E). The recombinant protein was affinity purified.

Recombinant Recombinant human complex of RBx1 (ring-box 1), CUL4B (cullin 4B), DBB1 (damage specific DNA binding protein 1), DCAF15 (DDB1 and CUL4 associated factor 15), and DDA1 (DET1 and DDB1 associated 1). RBx1, full length, encompassing amino acids 2-108(end). This construct contains an N-terminal His-tag (6xHis). CUL4B, encompassing amino acids 3-913(end). This construct contains an N-terminal His-tag (6xHis). DBB1, full length, encompassing amino acids 2-1140(end). This construct contains an N-terminal Flag-tag. DCAF15, full length, encompassing amino acids 2-600(end). This construct contains an N-terminal Flag-tag. DDA1, full length, encompassing amino acids 2-102(end). This construct contains an N-terminal Flag-tag. These recombinant proteins are co-expressed in a HEK293 expression system and affinity purified.

Recombinant Recombinant human complex of RBx1 (ring-box 1), CUL4B (cullin 4B), DBB1 (damage specific DNA binding protein 1), DCAF15 (DDB1 and CUL4 associated factor 15), and DDA1 (DET1 and DDB1 associated 1). RBx1, full length, encompassing amino acids 2-108(end). This construct contains an N-terminal His-tag (6xHis). CUL4B, encompassing amino acids 3-913(end). This construct contains an N-terminal His-tag (6xHis). DBB1, full length, encompassing amino acids 2-1140(end). This construct contains an N-terminal Flag-tag. DCAF15, full length, encompassing amino acids 2-600(end). This construct contains an N-terminal Flag-tag. DDA1, full length, encompassing amino acids 2-102(end). This construct contains an N-terminal Flag-tag. These recombinant proteins are co-expressed in a HEK293 expression system and affinity purified.

Recombinant human full length PKMYT1 (membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase), amino acids 2-500(end). This construct contains a N-terminal GST-tag, followed by a thrombin cleavage site. This protein was affinity purified.

Recombinant human TGFβR1 (transforming growth factor, beta receptor 1), also known as ALK5, encompassing amino acids 80-end. This construct contains an N-terminal GST tag. The recombinant protein was affinity purified and is kinase active.

GenCRISPR™ Cas13a (C2c2) Nuclease,1mg

GenCRISPR™ Cas12a (Cpf1) Nuclease,1mg

GenCRISPR™ Cas12a (Cpf1) Nuclease,500μg