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Inorganic pyrophosphate (PPi) is generated as a reaction byproduct in many biosynthetic reactions which utilize ATP, including in vitro transcription and DNA polymerization. Inorganic pyrophosphatase (PPase) catalyzes the hydrolysis of inorganic pyrophosphate to orthophosphate (P<sub>2</sub>O<sub>7</sub><sup>-4</sup> + H<sub>2</sub>O + PPase 2HPO<sub>4</sub><sup>-2</sup>). PPase requires divalent metal cation (Mg<sup>2+</sup>) for the enzymatic activity.