Labelling

MB 660R is a bright and photostable far-red dye that emits fluorescence at about 685 nm in the borderline spectral region between far-red and near-IR. Although the absorption maximum is at around 665 nm, this dye can be sufficiently excited by the 633 or 635 nm laser. MB 660R dye is water soluble and pH-insensitive from pH 4 to pH 10. MB 660R is a rhodamine-based dye, and like rhodamine dyes in general, it is very bright and exceptionally photostable (Figure 1). The superior photostability and excellent brightness of MB 660R makes this dye an ideal choice for confocal microscopy single molecule imaging and other applications that demand both brightness and photostability.

Rhodamine B NHS ester is a fluorophore featuring an NHS ester. Rhodamine B is a fluorescent dye which is used in fluorescence-based assays in vivo. NHS esters are highly reactive towards amines, forming amides under mild conditions.

DSPE-CH2-PEG-Fluor 488, MW 2,000 is a PEG lipid featuring a DSPE phospholipid and a Fluor 488 dye. DSPE is a phospholipid that is known to spontaneously form micelles in water and is featured heavily in lipid nanoparticles and drug development, while Fluor 488 is a cyanine dye that is prominently used in fluorescence microscopy. Fluor 488 has excitation and emission maxima at 499 nm and 520 nm.

FAM phosphoramidite, 5-isomer is a standard fluorescein (FAM) phosphoramidite for 5'-terminal oligonucleotide labeling, high isomeric purity single isomer. This highly purified amidite reagent ensures excellent coupling results with various synthesizers.

FAM phosphoramidite, 6-isomer is a standard fluorescein (FAM) phosphoramidite for 5'-terminal oligonucleotide labeling, high isomeric purity single isomer. This highly purified amidite reagent ensures excellent coupling results with various synthesizers.

This phosphoramidite allows fluorescein (FAM) to be introduced into any position in the oligonucleotide sequence during synthesis by the phosphoramidite method (in the middle of the chain, at the 5'- and 3'-terminus). The reagent is a conjugate of deoxythymidine phosphoramidite and 6-isomer of FAM.

Cyanine3 phosphoramidite, 5'-terminal is a fluorophore featuring a Cy3 dye ligated to a phosphoramidite group. This phosphoramidite is applied in solid-phase oligonucleotide synthesis to add a 5’-Cy3 dye to the target oligonucleotide. Cy3 is a cyanine dye with excitation and emission maxima at 555 nm and 570 nm respectively.

Cyanine5 is one of the dyes that are used in qPCR. The corresponding filter set is found on many qPCR machines.This reactive derivative is useful for the synthesis of qPCR probes having Cyanine5 at 5'-end, the most typical location of fluorophore. This is a terminating, non-nucleoside reagent.

Cyanine5.5 is a fluorophore with the emission in far red range of the spectrum. This dye is useful for multiplex qPCR. Commercial six-channel qPCR instruments often have a channel for Cyanine5.5.