Labelling

After the condensation of the trebler, three DNA branches begin to grow simultaneously with each step of the synthesis. Deblock of this construct gives rise to DNA containing a branching point. One arm (stem) is attached to the branch point with its 5'-end, and other arms (branches) are attached with their 3'-ends. Reverse amidites can be used to prepare constructs with different branch orientations.

Perylene is a bright and extremely photostable fluorescent polycyclic aromatic hydrocarbon (PAH) label with a quantum yield approaching quantitative. Due to the low lifetime of fluorescence, this probe does not form excimers.With this phosphoramidite, perylene can be introduced into DNA by automated oligonucleotide synthesis.

BP Lipid 802 is an ionizable lipid analogue of ALC-0315 featuring an ethanolamine head and two ester tails. One ester tail is an octanoate ester linked to a branched C17 tail. The other is a propanoate ester linked to a PEG3-undecyl tail. Ionizable lipids such as this may be applied in the development of lipid nanoparticles for drug delivery. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Methyltetrazine-activated Cy7 probe that reacts with TCO-containing compounds via an Inverse-Electron-Demand Diels-Alder reaction to form a stable covalent bond and does not require Cu-catalyst or elevated temperatures. The inverse-electron demand Diels-Alder cycloaddition reaction of TCO with tetrazines is a bioorthogonal reaction that possesses exceptional kinetics (k > 800 M-1 s -1) and selectivity. Such excellent reaction rate constants are unparalleled by any other bioorthogonal reaction pair described to date.

BP Fluor 532 Alkyne is a bright, yellow-fluorescent, alkyne-activated probe that reacts with azides via a copper-catalyzed click reaction (CuAAC). This probe is water-soluble and its fluorescence is pH independent over a wide pH range. The brightness and photostability of BP Fluor 532 dyes are well suited to direct imaging of low-abundance targets. The absorption/emission spectra of BP Fluor 532 is a perfect match to spectra of many other fluorescent dyes based on sulfonated rhodamine core, including Alexa Fluor® 532 and CF®532 Dye. For application where the presence of copper is not acceptable, please consider our BP Fluor™ 532 DBCO probe for copper-less detection of azide-modified molecules.

Azido Palmitic Acid (also provided as Click-iT® palmitic acid, azide) can be used to identify and characterize post-translationally palmitylated proteins with using a simple and robust two-step labeling and detection technique. Due to the very small size of the azide group and its complete absence in any natural molecules the presence of azide group does not affect the biodistribution of azido palmitic acid in comparison to the natural palmitic acid making it a suitable substrate for enzymes.

BP Fluor 488 TCO reacts with tetrazines to produce a stable, covalent linkage, also referred to as the inverse-electron demand Diels-Alder cycloaddition reaction. This reaction is extremely fast (k > 800 M-1 s-1), selective, biocompatible, and does not require Cu-catalyst or elevated temperatures. Such excellent reaction rate constants are unparalleled by any other bioorthogonal reaction pair described to date.

BP Fluor 568 TCO reacts with tetrazines to produce a stable, covalent linkage, also referred to as the inverse-electron demand Diels-Alder cycloaddition reaction. This reaction is extremely fast (k > 800 M -1 s-1), selective, biocompatible, and does not require Cu-catalyst or elevated temperatures. Such excellent reaction rate constants are unparalleled by any other bioorthogonal reaction pair described to date.

BP Fluor 594 TCO reacts with tetrazines to produce a stable, covalent linkage, also referred to as the inverse-electron demand Diels-Alder cycloaddition reaction. This reaction is extremely fast (k > 800 M -1 s-1), selective, biocompatible, and does not require Cu-catalyst or elevated temperatures. Such excellent reaction rate constants are unparalleled by any other bioorthogonal reaction pair described to date.