Labelling

ER Flipper-TR™ is a fluorescent probe that specifically targets the endoplasmic reticulum (ER) of cells and reports membrane tension changes through its fluorescence lifetime changes. It contains an ER targeting motive as well as a Flipper fluorophore which senses changes of the organization of lipid bilayer membranes through changes of the twist angle and polarization between the two twisted dithienothiophenes of the mechanophore. ER Flipper-TR™ spontaneously labels the ER of cells and is only fluorescent when inserted in a lipid membrane. It has a broad absorption and emission spectrum, excitation can be commonly performed with a 488nm laser, while emission is collected between 575 and 625nm.

Lyso Flipper-TR is a fluorescent probe that specifically targets the membrane of lysosomes of cells and reports membrane tension changes through its fluorescence lifetime changes. It contains a lysosome targeting motive as well as a Flipper fluorophore which senses changes of the organization of lipid bilayer membranes through changes of the twist angle and polarization between the two twisted dithienothiophenes of the mechanophore. Lyso Flipper-TR spontaneously labels lysosomes of cells and is only fluorescent when inserted in a lipid membrane. It has a broad absorption and emission spectrum, excitation can be commonly performed with a 488nm laser, while emission is collected between 575 and 625nm.

Mito Flipper-TR™ is a fluorescent probe that specifically targets the membrane of mitochondria of cells and reports membrane tension changes through its fluorescence lifetime changes. It contains a mitochondria targeting motive as well as a Flipper fluorophore which senses changes of the organization of lipid bilayer membranes through changes of the twist angle and polarization between the two twisted dithienothiophenes of the mechanophore. Mito Flipper-TR™ spontaneously labels mitochondria of live cells and is only fluorescent when inserted in a lipid membrane. It has a broad absorption and emission spectrum, excitation can be commonly performed with a 488nm laser, while emission is collected between 575 and 625nm.

SPY505-DNA is a bright green & non toxic live cell nuclear stain based on SPY™ dyes series. Its optimized structure allows quick labeling of DNA in live & fixed cells with high specificity and very low background. SPY505-DNA stains the nuclei of live or fixed cells without the need for genetic manipulation or overexpression of fluorescent proteins. Its absorbance and emission are similar to fluorescein or Alexa488™. SPY505-DNA enables multicolor imaging with SPY555, SPY595, SPY650, SPY700, SiR, RFP or mcherry. SPY505-DNA can be imaged with standard fluorescein (FITC) or YFP filtersets. It can be used for widefield, confocal, SIM or STED imaging in living or fixed cells and tissue. 

SPY555-DNA is a bright & non toxic live cell nuclear stain based on SPY™ dyes series. Its optimized structure allows quick labeling of DNA in live & fixed cells with high specificity and very low background. SPY555-DNA stains the nuclei of live or fixed cells without the need for genetic manipulation or overexpression of fluorescent proteins. Its absorbance and emission are similar to tetramethyl-rhodamine (TMR). SPY555-DNA enables multicolor imaging with SPY505, SPY595, SPY650, SPY700, SIR and GFP. SPY555-DNA can be imaged with standard TMR or Cy3 filtersets. It can be used for widefield, confocal, SIM or STED imaging in living or fixed cells and tissue.

SPY555-BG is the benzylguanine derivative of SPY555 fluorophore. It emits light in the orange part of the UV-ViS spectrum, it is fluorogenic, highly cell permeable and well suited for STED and SIM superresolution imaging. SPY555-BG can be imaged with a standard Cy3 filterset. It can be used for widefield, confocal, SIM or STED imaging in living or fixed cells and tissue. It has the same structure as MaP555-SNAP the name used in its original publication in Nature Chemistry. SPY555-BG is an alternative to SNAP-Cell®* TMR-Star, it has faster labeling kinetics. Benzylguanine (BG) is the substrate of the self labeling tag SNAP-tag™*. Upon reaction with a BG derivative, SNAP-tag™* forms a covalent bond with the substrate and releases guanine. It allows to permanently attach a fluorescent label to any protein of interest (POI) expressed as SNAP-tag™* fusion. *SNAP-tag™ and SNAP-Cell® are registered trademarks of New England Biolabs, Inc.

SPY595-DNA is a bright, photostable, red & non toxic live cell nuclear stain based on SPY™ dyes series. Its optimized structure allows quick labeling of DNA in live & fixed cells with high specificity and very low background. SPY595-DNA stains the nuclei of live or fixed cells without the need for genetic manipulation or overexpression of fluorescent proteins. Its emission in the red minimizes phototoxicity and sample autofluorescence. SPY595-DNA enables multicolor imaging with SPY505, SPY555, SPY650, SPY700, SiR and GFP. SPY595-DNA can be imaged with standard Alexa594™ or Texas Red filtersets. It can be used for widefield, confocal, SIM or STED imaging in living or fixed cells and tissue.

SPY620-DNA is a bright, photostable, red & non toxic live cell nuclear stain based on our SPY™ dyes series. Its optimized structure allows quick labeling of DNA in live & fixed cells with high specificity and very low background. SPY620-DNA stains the nuclei of live or fixed cells without the need for genetic manipulation or overexpression of fluorescent proteins. Its emission in the red minimizes phototoxicity and sample autofluorescence. SPY620-DNA enables multicolor imaging with SPY505, SPY555, SPY650, SPY700, SiR and GFP. SPY620-DNA can be imaged with e.g. using 610/20 nm and 650/40 nm excitation and emission filters respectively. It can be used for widefield, confocal, SIM or STED imaging in living or fixed cells and tissue.

SPY620-actin is a bright red & non toxic live and fixed cells F-actin stain based on SPY™ dyes series. Its optimized structure allows quick labeling of F-actin in live & fixed cells with high specificity and very low background. SPY620-actin is identical to MAP618-actin described in the nature chemistry article by the lab of Kai Johnsson. SPY620-actin stains F-actin of live or fixed cells without the need for genetic manipulation or overexpression of fluorescent proteins. Its emission in the red minimizes phototoxicity and sample autofluorescence. SPY620-actin enables multicolor imaging with SPY505, SPY555, SPY650, SPY700, SiR & GFP. SPY620-actin can be imaged with e.g. using 610/20 nm and 650/40 nm excitation and emission filters respectively. It can be used for widefield, confocal, SIM or STED imaging in living or fixed cells and tissue.