Labelling

Our potent and selective hOT receptor fluorescent antagonist shows high affinity for hOT receptor (Kd =1,59 nMin TR-FRET saturation binding assay). It allows to perform cell visualization in fluorescence microscopy, confocal microscopy, high content system experiments and TR-FRET experiments. It is potentially suitable for other fluorescence-based assays.

bis-Fluorescein-PEG8 is a fluorescein dye with excitation/emmission maximum 494/517 nm. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Fluorescein-PEG8-NHS ester is a hydrophilic fluorophore containing a NHS ester group and a hydrophilic PEG spacer arm. The NHS ester can react specifically and efficiently with primary amines such as the side chain of lysine residue or aminosilane-coated surfaces at neutral or slight basic conditions to form a covalent bond. The hydrophilic PEG spacer arm increases water solubility and conjugation efficiency. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Halo-Flipper is a fluorescent probe that specifically labels Halo-tag™* and reports membrane tension changes through its fluorescence lifetime changes. It contains a Chloroalcane Halo-tag™* ligand as well as a tethered Flipper fluorophore which senses changes of the organization of lipid bilayer membranes surrounding the Halo-tag™* protein . Halo-Flipper spontaneously labels Halotag expressing cells and is only fluorescent when inserted in a lipid membrane. It has a broad absorption and emission spectrum, excitation can be commonly performed with a 488nm laser, while emission is collected between 575 and 625nm. It is the perfect tool to precisely localize the Flipper-TR membrane tension fluorophore within cells. ChloroAlkane (CA) is the substrate of the self labeling tag Halo-tag™*. Upon reaction with a CA derivative, Halo-tag™* forms a covalent bond with the substrate. It allows to permanently attach a fluorescent label to any protein of interest (POI) expressed as Halo-tag™* fusion.

SPY505-CA is the ChloroAlkane (CA) derivative of our SPY505 fluorophore. It emits light in the green part of the UV-ViS spectrum, it is fluorogenic, highly cell permeable and well suited for STED and SIM superresolution imaging. SPY505-CA can be imaged with a standard FITC or YFP filterset. It can be used for widefield, confocal, SIM or STED imaging in living or fixed cells and tissue. It has the same structure as MaP510-halo, the name used in its original publication in Nature Chemistry. ChloroAlkane (CA) is the substrate of the self labeling tag Halo-tag™*. Upon reaction with a CA derivative, Halo-tag™* forms a covalent bond with the substrate. It allows to permanently attach a fluorescent label to any protein of interest (POI) expressed as Halo-tag™* fusion. Contains 1 vial of SPY505-CA (lyophilized). *Halo-tag™ is a registered trademark of Promega Corporation.

SPY555-CA is the ChloroAlkane (CA) derivative of our SPY555 fluorophore. It emits light in the orange part of the UV-ViS spectrum, it is fluorogenic, highly cell permeable and well suited for STED and SIM superresolution imaging. SPY555-CA can be imaged with a standard Cy3 filterset. It can be used for widefield, confocal, SIM or STED imaging in living or fixed cells and tissue. It has the same structure as MaP555-halo or MaP555-CA, the name used in its original publication in Nature Chemistry. ChloroAlkane (CA) is the substrate of the self labeling tag Halo-tag™*. Upon reaction with a CA derivative, Halo-tag™* forms a covalent bond with the substrate. It allows to permanently attach a fluorescent label to any protein of interest (POI) expressed as Halo-tag™* fusion. Contains 1 vial of SPY555-CA (lyophilized) *Halo-tag™ is a registered trademark of Promega Corporation.

SPY555-golgi is a bright, Orange & non toxic live cell golgi probe based on our SPY™ dyes series. Its optimized structure allows quick labeling of the golgi apparatus in live cells with high specificity and low background. SPY555-golgi stains the golgi apparatus in live cells without the need for genetic manipulation or overexpression of fluorescent proteins. Its absorbance and emission spectra are similar to TMR. SPY555-golgi enables multicolor imaging with SPY505, SPY595, SPY650, SPY700, SiR and GFP. SPY555-golgi can be imaged with standard TMR or Cy3 filtersets. It can be used for confocal, SIM or STED imaging in living cells and tissue. Contains 1 vial of SPY555-golgi (lyophilized).

CA_TestKit combines our 3 Halo-tag™ subtrates SPY505-CA, SPY555-CA and SiR-CA, offering a wide flexibility for Halo-tag™ fusion proteins labeling in live and fixed cells. The ligands are all bright, highly fluorogenic, and cell permeable. The 3 fluorophores are covering 3 distinct and very common imaging channels: FITC, TMR/Cy3 and Cy5. Kit contains 3 separate tubes containing 3 nmol of each Halo-tag™ substrate (ca. 30 stainings/tube). Halo-tag™ is a registered trademark of Promega Corporation.

SiR-CA is the ChloroAlkane (CA) derivative of our SiR fluorophore. It emits light in the far red part of the UV-ViS spectrum, it is fluorogenic, highly cell permeable and well suited for STED and SIM superresolution imaging. SiR-CA can be imaged with a standard Cy5 filterset. It can be used for widefield, confocal, SIM or STED imaging in living or fixed cells and tissue. It has the same structure as SiR-Halo, the name used in its original publication in Nature Chemistry. ChloroAlkane (CA) is the substrate of the self labeling tag Halo-tag™*. Upon reaction with a CA derivative, Halo-tag™* forms a covalent bond with the substrate. It allows to permanently attach a fluorescent label to any protein of interest (POI) expressed as Halo-tag™* fusion. Contains 1 vial of SiR-CA (lyophilized). *Halo-tag™ is a registered trademark of Promega Corporation.