Labelling

Alkyne-dATP (7-deaza-7-Ethynyl-dATP) is easily incorporated into DNA by PCR instead of dATP using polymerases like Pwo, Deep Vent exo- or KOD XL. The resulting stable and modified PCR fragment is now ready for further functionalization by Click Chemistry. We recommend a substitution of 1 – 10% of dATP.

Pseudo Uridine modified mRNA exhibits longer half-life, a better translation efficiency and its immunological properties are improved.

Pseudo Uridine modified mRNA exhibits longer half-life, a better translation efficiency and its immunological properties are improved.

Anti Reverse Cap Analog (ARCA) modified mRNA exhibits a longer half-life and the translation efficiency is increased by using this cap analog used during in vitro transcription for the generation of capped transcripts.

Anti Reverse Cap Analog (ARCA) modified mRNA exhibits a longer half-life and the translation efficiency is increased by using this cap analog used during in vitro transcription for the generation of capped transcripts.

Azide modified nucleotide closely resemble natural nucleotides and are the basis of chemoenzymatic mRNA labeling. Due to the small size of the azide this nucleotide is well accepted by T7 RNA polymerase and poly(A) polymerase and terminale deoxynucleotidyl transferase (Tdt) for enzymatic incorporation. This is highly beneficial, as labeling of mRNA with our approach is modular and not limited to enzymatic compatibility of bulky pre-labeled nucleotides. Moreover, only a very limited number of such pre-modified nucleotides, mainly dye-labeled ones, are commercially available. Internal and external studies successfully showed e.g. that the poly(A) polymerase adds specifically and sequence independently single azido terminator to the 3’-end of RNA. The main advantage: Any mRNA can be site-specifically labeled without special requirements or altered production protocols at the 3’-end.

Azide modified nucleotide closely resemble natural nucleotides and are the basis of chemoenzymatic mRNA labeling. Due to the small size of the azide this nucleotide is well accepted by T7 RNA polymerase and poly(A) polymerase and terminale deoxynucleotidyl transferase (Tdt) for enzymatic incorporation. This is highly beneficial, as labeling of mRNA with our approach is modular and not limited to enzymatic compatibility of bulky pre-labeled nucleotides. Moreover, only a very limited number of such pre-modified nucleotides, mainly dye-labeled ones, are commercially available. Internal and external studies successfully showed e.g. that the poly(A) polymerase adds specifically and sequence independently single azido terminator to the 3’-end of RNA. The main advantage: Any mRNA can be site-specifically labeled without special requirements or altered production protocols at the 3’-end.

Azide modified nucleotide closely resemble natural nucleotides and are the basis of chemoenzymatic mRNA labeling. Due to the small size of the azide this nucleotide is well accepted by T7 RNA polymerase and poly(A) polymerase and terminale deoxynucleotidyl transferase (Tdt) for enzymatic incorporation. This is highly beneficial, as labeling of mRNA with our approach is modular and not limited to enzymatic compatibility of bulky pre-labeled nucleotides. Moreover, only a very limited number of such pre-modified nucleotides, mainly dye-labeled ones, are commercially available. Internal and external studies successfully showed e.g. that the poly(A) polymerase adds specifically and sequence independently single azido terminator to the 3’-end of RNA. The main advantage: Any mRNA can be site-specifically labeled without special requirements or altered production protocols at the 3’-end.

Azide modified nucleotide closely resemble natural nucleotides and are the basis of chemoenzymatic mRNA labeling. Due to the small size of the azide this nucleotide is well accepted by T7 RNA polymerase and poly(A) polymerase and terminale deoxynucleotidyl transferase (Tdt) for enzymatic incorporation. This is highly beneficial, as labeling of mRNA with our approach is modular and not limited to enzymatic compatibility of bulky pre-labeled nucleotides. Moreover, only a very limited number of such pre-modified nucleotides, mainly dye-labeled ones, are commercially available. Internal and external studies successfully showed e.g. that the poly(A) polymerase adds specifically and sequence independently single azido terminator to the 3’-end of RNA. The main advantage: Any mRNA can be site-specifically labeled without special requirements or altered production protocols at the 3’-end.