Labelling

MagicLink&trade; NHS-PEG4-Biotin is a long (29.0&#197;), pegylated, water-soluble, NHS-ester biotinylation reagent to label amine-containing macromolecules and maximize solubility of antibodies and other proteins. The N-hydroxysuccinimide ester (NHS) group reacts specifically and efficiently with lysine and N-terminal amino groups to form stable amide bonds. The hydrophilic polyethylene glycol (PEG) spacer arm imparts water solubility that is transferred to the biotinylated molecule, thus reducing aggregation of labeled proteins stored in solution. The PEG spacer arm also gives the reagent a long and flexible connection to minimize steric hindrance for binding to avidin molecules. <br>Features: <ul style="list-style-type: disc"><li><strong>Amine-reactive:</strong> reacts with primary amines (-NH2)</li><li><strong>Pegylated spacer arm: </strong>hydrophilic polyethylene glycol (PEG) spacer arm (29 ), this reduces steric hindrance when binding to avidin molecules</li> <li><strong>Protein

MagicLink&trade; Sulfo-NHS-Biotin is a water-soluble reagent for simple and efficient biotin labeling of antibodies, proteins and other primary amine-containing macromolecules.<br /> Sulfo-NHS-Biotin is the shortest arm length (13.5 ) of three MagicLink&trade; biotinylation reagent which is water-soluble, non-cleavable, and enable simple and efficient biotinylation of antibodies, proteins and any other primary amine-containing macromolecules in solution. Specific labeling of cell surface proteins is another common application for these uniquely water-soluble and membrane impermeable reagents. Differing only in their spacer arm lengths, the Sulfo-NHS-ester reagents offer the possibility of optimizing labeling and detection experiments where steric hindrance of biotin binding is an important factor. Sulfo-NHS-Biotin is offered in separate package sizes and as complete protein labeling kits.AU60 <Br>Features: <ul style="list-style-type: disc"><li><strong>Amine-reactive:</strong> reacts wi

BroadPharm MagicLink&trade; Sulfo-NHS-LC-Biotin is an intermediate-length, water-soluble biotinylation reagent for labeling antibodies, proteins and other molecules that have primary amines. Features: <ul style="list-style-type: disc"><li><strong>Amine-reactive:</strong> reacts with primary amines (-NH2), such as lysine side-chains, or the amino-termini of polypeptides</li><li><strong>Soluble charged:</strong> sulfo-NHS group increases reagent water solubility compared to ordinary NHS-ester compounds</li><li><strong>Medium length spacer arm:</strong> (total length added to target) is 22.4 angstroms; provides excellent balance between adequate length (minimal steric hindrance for biotin binding) and modest mass</li><li><strong>Irreversible forms:</strong> permanent amide bonds; spacer arm cannot be cleaved</li><li><strong>Cell surface:</strong> labeling biotinylates only surface proteins of whole cells because the negatively charged reagent does not permeate cell membranes</li><li><stro

Streptavidin-HRP (1 vial)

This kit is an all-in-one kit that allows visualization of the endocytosis uptake of a target substance. The NH2-Reactive AcidSensor (fluorescent probe) included in the kit has an intramolecular active ester group that forms a stable covalent bond when mixed with an amino group-containing target substance (protein). The AcidSensor label can be excited at 633 nm, allowing for multiple staining with green or red fluorescence.

This kit includes lysosome staining dyes, pHLys Red (pH dependent), and LysoPrime Green (pH-independent). The pHLys Red and LysoPrime Green accumulate in the intact lysosomes. The fluorescence intensity of pHLys Red is enhanced as the acidity increases, and weak fluorescence is observed when lysosomes are neutralized due to the lysosomal dysfunction. On the other hand, LysoPrime Green gives stable emissions even lysosomes are neutralized. Lysosomal pH and lysosomal mass can be measured by combining these two dyes.

SY-9 (QSY®-9 equivalent) is a more hydrophilic version of the popular SY-7 dark quencher (QSY®-7 equivalent). This nonfluorescent acceptor dye is often used for the preparation of peptide FRET probes. A broad and intense quenching range from 500-600 nm makes it useful as an acceptor in fluorescence resonance energy transfer (FRET) applications in conjunction with dyes emitting at 500 nm to 600 nm, such as Fluorescein, Oregon Green, Carboxyrhodamine 110 (Rhodamine Green), Alexa Fluor® 488, 532, 546, 555, 568, Cy3, TAMRA and ROX dyes. SY-9 DBCO reacts with azides via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures. QSY®-9 is registered trademarks of ThermoFisher Scientific.

・Accurate pH changes can be detected by measuring lysosomal pH and volume changes in the same sample ・All-in-one Kit with Lysosome Acidification Inhibitor included ・Compatible with imaging and quantitative assay