Functional Assays

The Molecular Glue/PROTAC® Optimization Kit for CDK2/CDK9-Cereblon Binding is designed for the testing and profiling of Molecular Glues (MG) and PROTACs targeting CDK2 (cyclin dependent kinase 2) or CDK9 (cyclin dependent kinase 9) and Cereblon (CRBN). The Molecular Glue/PROTAC® Optimization Kit for CDK2/CDK9-Cereblon Binding comes in a convenient AlphaLISA® format, with enough PROTAC® buffer, purified CDK2/CyclinA2 and CDK9/CyclinK complexes, and CRBN for 384 reactions. This kit also contains the control PROTAC® (CDK2/9 Degrader) and CDK inhibitor FN-1501.The Molecular Glue/PROTAC® of interest is incubated with Cereblon (CRBN) and CDK2 or CDK9, bringing them into proximity. CRBN contains a FLAG-tag, which is recognized by anti-FLAG AlphaLISA„¢ acceptor bead. CDK contains a GST-tag that binds to the donor bead. Upon excitation of the donor bead, a singlet oxygen is generated by the bead. The singlet oxygen excites the acceptor bead, which emits light proportionally to the level of i

All-in-one kit for making phase-separated droplets with BSA (Bovine Serum Albumin), Droplets are easy to observe using only a micropipette and microscope, Includes a user's manual with calculation examples and details on how to use it

Set of reagents necessary to confirm the conditions for making phase-separated droplets, Crowding agent, pH, and salt can be optimized with this kit, Includes user's manual with sample calculations and methods for adjusting buffer pH

The iQuant™ microRNA Assay Kit is ideal for quantification of small RNA, including microRNA and siRNA, and both single stranded and double stranded RNA. The assay kit is highly selective for small RNA over rRNA or large mRNA. The assay kit offers advantages in stability, linear dynamic range, and sensitivity over other traditional of RNA quantitation. The assay kit contains concentrated quantitation reagent, dilution buffer, and pre-diluted microRNA standards. Simply dilute the reagent using the buffer provided, add your sample (any volume between 1 µl and 20 µl is acceptable), and read the fluorescence using fluorescence plate reader or Fluorometer (Figure 1). The assay is well tolerated to common contaminants such as proteins, salts, solvents, detergents, or free nucleotides. The assay can be adapted for use in microplates, tubes or cuvettes.