Cell Lysate

Jurkat cells were treated with PMA. Cells were solubilzed at 4 x 10⁶ cells/100µl in cell lysis buffer.

Jurkat cells were treated with PV (Pervanadate). Solubilize cells at 4 x 10⁶ cells/100µl in cell lysis buffer.

K562 cells were treated with Pervanadate at 37°C for 10 min. Solubilize cells at 4 x 10⁶ cells/100µl in lysis buffer.

T47D cells were treated with recombinant human IGF-1 at 37°C for 30 min. Cells were solubilzed at 4 x 10⁶ cells/100µl in cell lysis buffer.

T47D cells were exposed to 50J/m2 of UV light followed by a 4 hours recovery period. Cells were solubilzed at 4 x 10⁶ cells/100µl in cell lysis buffer.

T47D cells were treated with Pervanadate at 37°C for 10 min. Solubilize cells at 4 x 10⁶ cells/100µl in lysis buffer.

THP1 cells were treated with Pervanadate at 37°C for 10 min. Cells were solubilzed at 4 x 10⁶ cells/100µl in lysis buffer.

U-251 cells were treated with Pervanadate. Solubilize cells at 4 x 10⁶ cells/100µl in cell lysis buffer.

U937 cells were treated with Pervanadate at 37°C for 10 min. Cells were solubilzed at 4 x 10⁶ cells/100µl in lysis buffer.