Labelling

IR 650 DBCO is a fluroescent molecule with a terminal propargyl group. The DBCO groups is commonly used for copper-free Click Chemistry reactions due to its strain promoted high energy. It is a very hydrophilic, water-soluble, far-red fluorescent dye that has a maximal absorption of 651 nm and emission at 668 nm.

IR 680LT NHS ester is a fluorescent molecule with an NHS ester moiety. The NHS ester can be applied to label the primary amines (-NH2) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. It is a far-red, water-soluble dye that is structurally very similar to the commonly used Cy5.5 fluroescent dye.

IR 750 NHS ester is a near-red, water-soluble dye that is structurally similar to other 750 dyes which absorb at 756 nm and emit at 776 nm. The NHS ester can be applied to label the primary amines (-NH2) of proteins, amine-modified oligonucleotides, and other amine-containing molecules.

IR 750 Maleimide is a near-red, water-soluble dye that is structurally similar to other 750 dyes which absorb at 756 nm and emit at 776 nm. The maleimide group will react with a thiol group to form a covalent bond.

IR 750 DBCO is a fluroescent molecule with a terminal DBCO group. IR 750 DBCO is a near-red, water-soluble dye that is structurally similar to other 750 dyes which absorb at 756 nm and emit at 776 nm. The DBCO groups is commonly used for copper-free Click Chemistry reactions due to its strain promoted high energy.

6,8-Difluoro-7-hydroxy-4-methylcoumarin NHS Ester is a molecule with an excitation at 365 nm and emission at 460 nm. The NHS ester can be applied to label the primary amines (-NH2) of proteins, amine-modified oligonucleotides, and other amine-containing molecules.

Fmoc-Lys(AMCA)-OH is a fluroescent molecule with a Boc protecting group and terminal carboxylic acid and Fmoc moieties. The protected amine can be deprotected under mild acidic conditions. It has an excitation at 345 nm and emission at 450 nm with a relatively large Stoke’s shift and resistance to photobleaching. The terminal carboxylic acid can react with primary amine groups in the presence of activators (e.g. HATU) to form a stable amide bond. The Fmoc group can be deprotected under basic conditions to obtain the free amine which can be used for further conjugations.

Fmoc-Lys(CR110)-OH is a green fluroescent molecule with two Boc protecting groups and terminal carboxylic acid and Fmoc moieties. The protected amines can be deprotected under mild acidic conditions. It has an excitation at 502 nm and emission at 527 nm. The terminal carboxylic acid can react with primary amine groups in the presence of activators (e.g. HATU) to form a stable amide bond. The Fmoc group can be deprotected under basic conditions to obtain the free amine which can be used for further conjugations.

Sulfo-Cy3-amide-C6-maleimide is a water soluble, thiol-reactive dye for labeling with a hydrophilic sulfo-Cyanine3 fluorophore. The maleimide group will react with a thiol group to form a covalent bond.