ELISA Reagents

8 ml of Stop Solution, containing 0.2 M sulfuric acid

12 ml of 3,3',5,5'-tetramethylbenzidine (TMB) in buffered solution

MagicBrite ECL PicoPlus Substrate Kit is a sensitive, enhanced luminol-based chemiluminescent substrate (ECL) for the detection of horseradish peroxidase (HRP) in immunoblotting and other immunoassay applications. The ECL substrate detects low pico gram amounts of antigen with stable and long light output (up to 4 hours), which can be visualized using X-ray films or CCD-based imagers. Appropriate primary and secondary antibody dilutions are suggested for attaining optimal signal intensity and duration.<br /><br>Features:<br /><strong>Sensitivity:</strong> low picogram<br /> <strong>Stability:</strong> 1-hr working solution stability, 1-year kit stability at room temperature. <br /><strong>Compatibility:</strong> nitrocellulose and PVDF membranes<br /> <strong>Signal duration:</strong> up to 4 hours<br /> <strong>Recommended dilution:</strong> primary antibody: 1:1,000 1:5,000; secondary antibody: 1:20,000 to 1:100,000