Lab Tools

HDAC assay buffer is an essential reagent for signal generation in HDAC assays performed with any of BPS Bioscience's HDAC fluorimetric substrates or assay kits. An appropriate fluorimetric substrate, comprising an acetylated lysine side chain, is first incubated with a sample containing HDAC activity (nuclear or cellular extract, purified enzyme, bead-bound immunocomplex, etc.) in HDAC assay buffer. Deacetylation of the substrate sensitizes it so that, in a second step, treatment with the HDAC assay developer produces a fluorophore. Trichostatin A is included asan 'inhibitor stop' for class I and II HDACs.

Aqueous buffer optimized for use with BPS Bioscience's purified sirtuin (SIRT) enzymes and SIRT assay kits.

Buffer optimized for use with BPS Bioscience's histone acetyltransferase (HAT) assay kits.

Concentrated aqueous buffer optimized for use with the following BPS Bioscience HSP90 assay kits: <br />• HSP90α Assay Kit (96 well) #50293 96 reactions <br />• HSP90α Assay Kit (384 well) #50298 384 reactions <br />• HSP90β Assay Kit (96 well) #50294 96 reactions <br />• HSP90β Assay Kit (384 well) #50299 384 reactions <br />Note: This buffer is not designed for use with the HSP90β (C-Terminal) Inhibitor Screening Assay Kit, Cat. # 50314.

Concentrated aqueous buffer optimized for use with the following BPS Bioscience HSP90 assay kits: • HSP90α (C-Terminal) Inhibitor Screening Assay Kit #50317 384 rxns • HSP90β (C-Terminal) Inhibitor Screening Kit #50314 384 rxns • HSP90α (C-Terminal Domain) TR-FRET Kit #50261 384 rxns • HSP90β (C-Terminal Domain) TR-FRET Kit #50262 384 rxns Note: This buffer is not designed for use with the HSP90 N-Terminal Assay Kits.

This Blocking Buffer 4 is optimized for minimizing background in various chemiluminescent methyltransferase, demethylase, and TET assays.

HMT Assay Buffer 1 is optimized for assaying G9a, MLL, Set7/9, SUV39H1, and SUV39H2. This buffer is designed for BPS Bioscience's direct activity chemiluminescent assays. For BPS Bioscience's homogeneous assays, use cat. #52160-A.

HMT Assay Buffer 2 is optimized for assaying EZH1, EZH2, PRMT1, PRMT3, and PRMT5. This buffer is designed for BPS Bioscience's direct activity chemiluminescent assays. For BPS Bioscience's homogeneous assays, use cat. #52170-A.

HMT Assay Buffer 3 is optimized for assaying DOT1L using BPS Bioscience's assay kit.