Buffers & Concentrates

The 3x BRD TR-FRET Assay Buffer 1 is optimized for use with the following BPS Bioscience assay kits: ATAD2A TR-FRET Assay Kit #32618 384 reactions ATAD2B TR-FRET Assay Kit #32620 384 reactions BPTF/FALZ TR-FRET Assay Kit #32632 384 reactions BRD1 TR-FRET Assay Kit #32625 384 reactions BRD2 (BD1+BD2) TR-FRET Assay Kit #32614 384 reactions BRD3 (BD1+BD2) TR-FRET Assay Kit #32615 384 reactions BRD4 (BD1+BD2) TR-FRET Assay Kit #32612 384 reactions BRD4 (BD1) TR-FRET Assay Kit #32613 384 reactions BRD4 (BD2) TR-FRET Assay Kit #32617 384 reactions BRD9 TR-FRET Assay Kit #32621 384 reactions BRD9 TR-FRET Assay Kit #32621 384 reactions BRPF3 TR-FRET Assay Kit #32626 384 reactions CECR2 TR-FRET Assay Kit #32622 384 reactions CREBBP TR-FRET Assay Kit #32619 384 reactions TAF1 (BD2) TR-FRET Assay Kit #32627 384 reactions TAF1 (BD1+BD2) TR-FRET Assay Kit #32628 384 reactions TAF1L (BD1+BD2) TR-FRET Assay Kit #32629 384 reactions WDR9/BRWD1 TR-FRET Assay Kit #32631 384 reactions

The 3x BRD TR-FRET Assay Buffer 2 is optimized for use with the following BPS Bioscience assay kits: • TRIM24 TR-FRET Assay Kit #32630 384 reactions

The 3x PCSK9 TR-FRET Assay Buffer is optimized for use with the following BPS Bioscience assay kits: • PCSK9-LDLR TR-FRET Assay Kit #72010 384 reactions • PCSK9(D374T)-LDLR TR-FRET Assay Kit #72011 384 reactions

HDAC assay buffer is an essential reagent for signal generation in HDAC assays performed with any of BPS Bioscience's HDAC fluorimetric substrates or assay kits. An appropriate fluorimetric substrate, comprising an acetylated lysine side chain, is first incubated with a sample containing HDAC activity (nuclear or cellular extract, purified enzyme, bead-bound immunocomplex, etc.) in HDAC assay buffer. Deacetylation of the substrate sensitizes it so that, in a second step, treatment with the HDAC assay developer produces a fluorophore. Trichostatin A is included asan 'inhibitor stop' for class I and II HDACs.

Aqueous buffer optimized for use with BPS Bioscience's purified sirtuin (SIRT) enzymes and SIRT assay kits.

Buffer optimized for use with BPS Bioscience's histone acetyltransferase (HAT) assay kits.

Concentrated aqueous buffer optimized for use with the following BPS Bioscience HSP90 assay kits: <br />• HSP90α Assay Kit (96 well) #50293 96 reactions <br />• HSP90α Assay Kit (384 well) #50298 384 reactions <br />• HSP90β Assay Kit (96 well) #50294 96 reactions <br />• HSP90β Assay Kit (384 well) #50299 384 reactions <br />Note: This buffer is not designed for use with the HSP90β (C-Terminal) Inhibitor Screening Assay Kit, Cat. # 50314.

Concentrated aqueous buffer optimized for use with the following BPS Bioscience HSP90 assay kits: • HSP90α (C-Terminal) Inhibitor Screening Assay Kit #50317 384 rxns • HSP90β (C-Terminal) Inhibitor Screening Kit #50314 384 rxns • HSP90α (C-Terminal Domain) TR-FRET Kit #50261 384 rxns • HSP90β (C-Terminal Domain) TR-FRET Kit #50262 384 rxns Note: This buffer is not designed for use with the HSP90 N-Terminal Assay Kits.

This Blocking Buffer 4 is optimized for minimizing background in various chemiluminescent methyltransferase, demethylase, and TET assays.