Buffers & Concentrates

CFSE is used for detection of viable cell as well as for the long-term observation of cell activities by fluorescent microscopy. The excitation and emission wavelengths of CFSE-labeled cells are 500 nm and 520 nm, respectively.

D-Luciferin K salt is a firefly luciferase substrate.

CUTANA™ Nuclei Extraction Buffer is the essential reagent for harvesting nuclei from cultured cells and tissues for use in CUT&RUN and CUT&Tag assays. This buffer is expected to be broadly compatible with eukaryotic cells and tissues. Below are examples of various cells and tissue types that have undergone successful nuclei isolation using this buffer: mouse NIH3T3 fibroblast cells human A549 non-small cell lung cancer (NSCLC) cells human K562 leukemia cells human NCI-H1299 non-small cell lung cancer (NSCLS cells) human bone marrow derived macrophages human TIG-1 fetal lung cells human monocyte derived macrophages human LoVo colorectal cancer cells human MV-4-11 macrophage cells human LNCaP prostate carcinoma cells human SUM149 triple negative breast cancer (TNBC) cells human renal primary cells human GM24385 B-lymphocyte (aka HG002) cells human peripheral blood mononuclear cells (PBMCs) human MCF7 breast cancer cells human intestinal tissue human MDA-MB-231 bre

Mouse-FREE IgG

The 10X Whole Blood Lysing Solution is commonly used for lysing red blood cells following direct immunofluorescence staining of human peripheral blood cells with monoclonal antibodies prior to flow cytometric analysis.

The 10X Whole Blood Lysing Solution is commonly used for lysing red blood cells following direct immunofluorescence staining of human peripheral blood cells with monoclonal antibodies prior to flow cytometric analysis.

PEG Blocking Buffer for ELISA contain polyoxyethylene detergents that interfere with PEG and PEG antibody detection. PEGB125-1 effectively blocks and stabilizes PEG-protein and anti-PEG coated ELISA plates if used as directed.

PEG Dilution Buffer contains an effective blocking agent that minimizes non specific binding and a non polyoxyethylene detergent that decreases variability and background.