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Benzyloxy carbonyl-PEG12-t-butyl ester is a heterobifunctional linker comprised of a benzyloxy carbonyl where the benzyl protecting group can be removed by hydrogenolysis and t-butyl ester can be deprotected under acidic conditions. The PEG12 chain improves the molecule's hydrophilicity.
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N-Mal-N-bis(PEG8-NH-Boc) serves as a branched PEG linker consisting of two Boc groups which can be removed under acidic conditions to free the amine for further reactions. The maleimide attached to the central amine of the molecule can react directly with thiol-bearing entities to form a covalent bond.