Nucleotides

This reagent is designed for the synthesis of oligonucleotides bearing triple bond (alkyne) in the middle of the chain. Alkyne is attached to deoxyuridine fragment, and it does not interfere with the hybridization to the complementary strand.

Phosphoramidite for the synthesis of oligonucleotides with 5'-terminal alkyne for click chemistry. This alkyne amidite has several advantages over 5’-hexynyl phosphoramidite, 5'-butynyl-CEP, and other 5'-terminal alkyne phosphoramidites.

Bicyclononyne (BCN) is a stable and one of the most reactive cyclooctynes for copper-free click chemistry. Unlike dibenzocyclooctyne (DBCO), BCN is reactive both to azides (strain-promoted azyde-alkyne cycloaddition, SPAAC) and tetrazines (inverse electron demand Diels-Alder reaction, IEDDA)

Chemical phosphorylation reagent for the synthesis of 5'-phosphorylated oligonucleotides. The reagent contains a DMT group in its structure, which allows oligonucleotide purification on C18-cartridges or by reversed-phase chromatography.

Non-nucleosidic modifier reagent for the synthesis of biotinylated oligos based on a hydroxyprolinol core. This phosphoramidite contains a dimethoxytrityl protective group for the purification of the resulting oligos on C18 cartridges, and reverse phase HPLC.

A compound for the phosphoramidite synthesis of oligonucleotides and the creation of the abasic step in the oligonucleotide sequence.Abasic phosphoramidites are used in DNA and oligonucleotide synthesis.

Spacer phosphoramidite 18 (hexaethylene glycol phosphoramidite) is an amidite reagent for oligonucleotide synthesis. It provides a long hydrophilic linker that can be used for the synthesis of Scorpion-type qPCR probes, for spacing biotin from oligo strand, and for other applications requiring spatial separation of modifier and oligo.

Pyrene is a polycyclic aromatic hydrocarbon that is well-known for its ability to intercalate into DNA. Pyrene features intense blue fluorescence. Its parameters depend strongly on the fluorophore microenvironment. Therefore, fluorescence spectra of pyrene are used for the extraction of structural information about the site surrounding pyrene.

NH-bis(PEG8-OH) is a homobifunctional reagent containing two alcohols joined together by a secondary amine. The alcohols can be used in a variety of ways such as in forming esters with carboxylic acids, while the secondary amine can be used as a nucleophile in linking with ketones, aldehydes, and carboxylic acids. The PEG spacers make this molecule water-soluble, potentially altering its DMPK properties.