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Recombinant SARS-CoV-2 Spike protein S1 subunit, encompassing amino acids 16-685. This protein contains three mutations of interest K417T, E484K and N501Y (found in the RBD domain of some variants of concern). The construct contains a C-terminal Avi-Tag™ followed by a His-tag (6xHis). The protein was enzymatically biotinylated using the Avi-Tag™ and affinity purified.
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Mouse FcRn also known as Neonatal Fc receptor is a heterodimer of FCGRT (Fc Fragment of IgG Receptor and Transporter) and B2M (beta 2 microglobulin), GenBank Accession numbers NM_010189 and NM_009735; a.a. 22-297 and a.a. 1-119 respectively. FCGRT has a C-terminal His-Avi-tag. These proteins are co-expressed in a HEK293 expression system. FcRn MW = 34 kDa. B2M MW=14 kDa.