Peptides & Amino Acids

Non-specific binding of an antibody to proteins other than the antigen can sometimes occur. This is usually more common with polyclonal antibodies, but can also occur with monoclonals as well. To determine which band or staining is specific, the AKT immunizing peptide blocking experiment can be performed. Prior to the staining protocol, the anti-AKT antibody is neutralized (incubated with an excess of peptide that corresponds to the epitope recognized by the antibody). The AKT antibody that is bound to the blocking peptide is no longer available to bind to the epitope present in the protein on the Western blot or in the cell. The neutralized antibody is then used side-by-side with the antibody alone, and the results are compared. By comparing the staining from the blocked antibody versus the antibody alone, you can see which staining is specific: this staining will be absent from the Western blot or immunostaining performed with the neutralized antibody.

This peptide is a control blocking and immunogen peptide for the cardiovascular angiopoietin antibodies 100-401-402 and 100-401-402S

This peptide is a control blocking and immunogen peptide for the cardiovascular angiopoietin antibodies 100-401-403 and 100-401-403S.

Osteopontin peptide for use in control assays with purified antibody Anti-Osteopontin (p/n 100-401-404).

Notch-1 (intra) peptide for use in control assays with purified antibody Anti-Notch-1 (p/n 100-401-405).

Notch-1 (cleaved N-term) peptide for use in control assays with purified antibody Anti-Notch-1 (p/n 100-401-407 and 100-401-407S).

Notch-2 (cleaved N-term) peptide for use in control assays with purified antibody Anti-Notch-2 (p/n 100-401-408 and 100-401-408S).

ASK-1 phospho S83 peptide for use in control assays with purified antibody Anti-ASK-1 pS83 (p/n 200-401-410). Also available ASK-1 non-phospho S83 peptide (p/n 000-000-410NP).

ASK-1 non-phospho S83 peptide for use in control assays with purified antibody Anti-ASK-1 pS83 (p/n 200-401-410). Also available ASK-1 phospho S83 peptide (p/n 000-000-410).