Lentivirus

Please note this product may be subject to fees, we invite you to contact your local office. Firefly Luciferase-mCherry Lentivirus (Hygromycin or Puromycin) are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles ready to transduce nearly all types of mammalian cells, including primary and non-dividing cells. These viruses contain firefly luciferase and mCherry (Luc2-P2A-mCherry) driven by an EF1a promoter, and a hygromycin or puromycin selection marker. Luciferase and mCherry are co-expressed under the EF1A promoter in cells, allowing for flexibility in the detection of transduced cells.

Please note this product may be subject to fees, we invite you to contact your local office. Firefly Luciferase-mCherry Lentivirus (Hygromycin or Puromycin) are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles ready to transduce nearly all types of mammalian cells, including primary and non-dividing cells. These viruses contain firefly luciferase and mCherry (Luc2-P2A-mCherry) driven by an EF1a promoter, and a hygromycin or puromycin selection marker. Luciferase and mCherry are co-expressed under the EF1A promoter in cells, allowing for flexibility in the detection of transduced cells.

LV-EF1α-NLS-GFP-Puro is a pre-made lentivirus which express eGFP fused with SV40 nuclear localization sequence (NLS) under the EF1α promoter with co-expression of puromycin selection in a IRES cassette. Ready to use format.

LV-EF1α-NLS-mCherry-Puro is a pre-made lentivirus which expresses mCherry fused with SV40 nuclear localization sequence (NLS) under the EF1α promoter with co-expression of puromycin selection in a IRES cassette. Ready to use format.

PD-L1 Lentiviruses are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles ready to transduce nearly all types of mammalian cells, including primary and non-dividing cells. These viruses contain human PD-L1 (programmed death ligand 1) (NM_014143.4) driven by an EF1a promoter and a geneticin selection marker.

MAGE-A1-Specific TCR Lentivirus (Clone 1367) are replication incompetent, HIV-based, VSV-G-pseudotyped lentiviral particles ready to transduce nearly all types of mammalian cells, including primary and non-dividing cells. These viruses transduce cells with a TCR (T cell receptor) (clone 1367) that specifically recognizes the human antigen MAGE-A1 (Melanoma-associated antigen 1), and in which the TCR α chain and β chain are linked by P2A. The lentiviruses also transduce a puromycin selection marker. Mouse TCR constant regions were used in this construct to boost expression.

MAGE-A1-Specific TCR Lentivirus (Clone 1367) are replication incompetent, HIV-based, VSV-G-pseudotyped lentiviral particles ready to transduce nearly all types of mammalian cells, including primary and non-dividing cells. These viruses transduce cells with a TCR (T cell receptor) (clone 1367) that specifically recognizes the human antigen MAGE-A1 (Melanoma-associated antigen 1), and in which the TCR α chain and β chain are linked by P2A. The lentiviruses also transduce a puromycin selection marker. Mouse TCR constant regions were used in this construct to boost expression.

MAGE-A4-Specific TCR Lentivirus are replication incompetent, HIV-based, VSV-G-pseudotyped lentiviral particles ready to transduce nearly all types of mammalian cells, including primary and non-dividing cells. These viruses transduce cells with a TCR (T cell receptor) that specifically recognizes the human antigen MAGE-A4 (Melanoma-associated antigen 4) peptide 230-239 (GVYDGREHTV), and in which the TCR α chain and β chain are linked by P2A. The lentiviruses also transduce a puromycin selection marker.