Lentivirus

MAGE-A4-Specific TCR Lentivirus are replication incompetent, HIV-based, VSV-G-pseudotyped lentiviral particles ready to transduce nearly all types of mammalian cells, including primary and non-dividing cells. These viruses transduce cells with a TCR (T cell receptor) that specifically recognizes the human antigen MAGE-A4 (Melanoma-associated antigen 4) peptide 230-239 (GVYDGREHTV), and in which the TCR α chain and β chain are linked by P2A. The lentiviruses also transduce a puromycin selection marker.

KRAS G12D-Specific TCR Lentivirus (Clone 9c) are replication incompetent, HIV-based, VSV-G-pseudotyped lentiviral particles ready to transduce nearly all types of mammalian cells, including primary and non-dividing cells. These viruses transduce cells with a TCR (T cell receptor) (clone 9c) that specifically recognizes the human antigen KRAS (Kirsten rat sarcoma virus) G12D, and in which the TCR α chain and β chain are linked by P2A. The lentiviruses also transduce a puromycin selection marker (Figure 1). Mouse TCR constant regions were used in this construct to boost expression.

KRAS G12D-Specific TCR Lentivirus (Clone 9c) are replication incompetent, HIV-based, VSV-G-pseudotyped lentiviral particles ready to transduce nearly all types of mammalian cells, including primary and non-dividing cells. These viruses transduce cells with a TCR (T cell receptor) (clone 9c) that specifically recognizes the human antigen KRAS (Kirsten rat sarcoma virus) G12D, and in which the TCR α chain and β chain are linked by P2A. The lentiviruses also transduce a puromycin selection marker (Figure 1). Mouse TCR constant regions were used in this construct to boost expression.

KRAS G12D-Specific TCR Lentivirus (Clone 10) are replication incompetent, HIV-based, VSV-G-pseudotyped lentiviral particles ready to transduce nearly all types of mammalian cells, including primary and non-dividing cells. These viruses transduce cells with a TCR (T cell receptor) (clone 10) that specifically recognizes the human antigen KRAS (Kirsten rat sarcoma virus) G12D, and in which the TCR α chain and β chain are linked by P2A. The lentiviruses also transduce a puromycin selection marker (Figure 1). Mouse TCR constant regions were used in this construct to boost expression.

KRAS G12D-Specific TCR Lentivirus (Clone 10) are replication incompetent, HIV-based, VSV-G-pseudotyped lentiviral particles ready to transduce nearly all types of mammalian cells, including primary and non-dividing cells. These viruses transduce cells with a TCR (T cell receptor) (clone 10) that specifically recognizes the human antigen KRAS (Kirsten rat sarcoma virus) G12D, and in which the TCR α chain and β chain are linked by P2A. The lentiviruses also transduce a puromycin selection marker (Figure 1). Mouse TCR constant regions were used in this construct to boost expression.

Membrane-Bound TNFα Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to transduce nearly all types of mammalian cells, including primary and non-dividing cells. These particles result in expression of uncleavable, membrane-bound TNFα (Tumor necrosis factor alpha) driven by an EF1a promoter, and a puromycin selection marker.

The Spike (BA.2.86, Omicron Variant) (SARS-CoV-2) Pseudotyped Lentivirus (Luciferase Reporter) are replication incompetent, HIV-based lentiviral particles. They were produced with SARS-CoV-2 Spike (Genbank Accession #QHD43416.1 containing all the BA.2.86 mutations; see below for details) as the envelope glycoprotein, instead of the commonly used VSV-G. These pseudovirions also contain the firefly luciferase reporter driven by a CMV promoter, allowing to measure spike-mediated cell entry using luciferase activity. These pseudoviruses have been validated in a cellular assay with ACE2-HEK293 Recombinant Cell Line (#79951), a cell line that overexpresses ACE2 at high levels, as target cell line.

The Spike (BA.2.86, Omicron Variant) (SARS-CoV-2) Pseudotyped Lentivirus (Luciferase Reporter) are replication incompetent, HIV-based lentiviral particles. They were produced with SARS-CoV-2 Spike (Genbank Accession #QHD43416.1 containing all the BA.2.86 mutations; see below for details) as the envelope glycoprotein, instead of the commonly used VSV-G. These pseudovirions also contain the firefly luciferase reporter driven by a CMV promoter (Figure 1), allowing to measure spike-mediated cell entry using luciferase activity. These pseudoviruses have been validated in a cellular assay with ACE2-HEK293 Recombinant Cell Line (#79951), a cell line that overexpresses ACE2 at high levels, as target cell line.

The Spike (JN.1, Omicron Variant) (SARS-CoV-2) Pseudotyped Lentivirus (Luciferase Reporter) are replication incompetent, HIV-based lentiviral particles. They were produced with SARS-CoV-2 Spike (Genbank Accession #QHD43416.1 containing all the JN.1 mutations; see below for details) as the envelope glycoprotein, instead of the commonly used VSV-G. These pseudovirions also contain a firefly luciferase reporter driven by a CMV promoter (Figure 1), allowing to measure spike-mediated cell entry using luciferase activity. These pseudoviruses have been validated in a cellular assay with ACE2-HEK293 Recombinant Cell Line (#79951), a cell line that overexpresses ACE2 at high levels, as target cell line.