Lentivirus

The Spike (JN.1, Omicron Variant) (SARS-CoV-2) Pseudotyped Lentivirus (Luciferase Reporter) are replication incompetent, HIV-based lentiviral particles. They were produced with SARS-CoV-2 Spike (Genbank Accession #QHD43416.1 containing all the JN.1 mutations; see below for details) as the envelope glycoprotein, instead of the commonly used VSV-G. These pseudovirions also contain a firefly luciferase reporter driven by a CMV promoter (Figure 1), allowing to measure spike-mediated cell entry using luciferase activity. These pseudoviruses have been validated in a cellular assay with ACE2-HEK293 Recombinant Cell Line (#79951), a cell line that overexpresses ACE2 at high levels, as target cell line.

The Spike (BA.2.86 Omicron Variant) (SARS-CoV-2) Pseudotyped Lentivirus (eGFP Reporter) are replication incompetent, HIV-based lentiviral particles. They were produced with SARS-CoV-2 Spike (Genbank Accession #QHD43416.1 containing all the Omicron BA.2.86 mutations; see below for details) as the envelope glycoprotein, instead of the commonly used VSV-G. These pseudovirions also contain the eGFP reporter driven by a CMV promoter (Figure 1), allowing to measure spike-mediated cell entry using the eGFP fluorescence signal. The pseudovirions have been validated in a cellular assay with ACE2-HEK293 Recombinant Cell Line (#79951), a cell line that overexpresses ACE2 at high levels, as target cell line.

The Spike (BA.2.86 Omicron Variant) (SARS-CoV-2) Pseudotyped Lentivirus (eGFP Reporter) are replication incompetent, HIV-based lentiviral particles. They were produced with SARS-CoV-2 Spike (Genbank Accession #QHD43416.1 containing all the Omicron BA.2.86 mutations; see below for details) as the envelope glycoprotein, instead of the commonly used VSV-G. These pseudovirions also contain the eGFP reporter driven by a CMV promoter (Figure 1), allowing to measure spike-mediated cell entry using the eGFP fluorescence signal. The pseudovirions have been validated in a cellular assay with ACE2-HEK293 Recombinant Cell Line (#79951), a cell line that overexpresses ACE2 at high levels, as target cell line.

The Spike (JN.1 Omicron Variant) (SARS-CoV-2) Pseudotyped Lentivirus (eGFP Reporter) are replication incompetent, HIV-based lentiviral particles. They were produced with SARS-CoV-2 Spike (Genbank Accession #QHD43416.1 containing all the Omicron JN.1 mutations; see below for details) as the envelope glycoprotein, instead of the commonly used VSV-G. These pseudovirions also contain an eGFP reporter driven by a CMV promoter, allowing to measure the spike-mediated cell entry using the eGFP fluorescence signal. The pseudovirions have been validated in a cellular assay with ACE2-HEK293 Recombinant Cell Line (#79951), a cell line that overexpresses ACE2 at high levels, as target cell line.

The Spike (JN.1 Omicron Variant) (SARS-CoV-2) Pseudotyped Lentivirus (eGFP Reporter) are replication incompetent, HIV-based lentiviral particles. They were produced with SARS-CoV-2 Spike (Genbank Accession #QHD43416.1 containing all the Omicron JN.1 mutations; see below for details) as the envelope glycoprotein, instead of the commonly used VSV-G. These pseudovirions also contain an eGFP reporter driven by a CMV promoter, allowing to measure the spike-mediated cell entry using the eGFP fluorescence signal. The pseudovirions have been validated in a cellular assay with ACE2-HEK293 Recombinant Cell Line (#79951), a cell line that overexpresses ACE2 at high levels, as target cell line.

LV-EF1a-Mito-GFP-IRES-Puro is a pre-made lentivirus which expresses Mito-EGFP under the EF1a promoter with the co-expression of the Puro resistance marker via the IRES element. The mitochondrial targeting sequence from subunit VIII of human cytochrome C oxidase (Mito) is fused with EGFP. The Mito sequence is fused to the 5'-end of EGFP, The Mito sequence targets the Mito-EGFP fusion protein to the host cell’s mitochondria.