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The Ovine Bead-based Immuno-Quantitative (BIQ) IL-1 beta/IL-1 F2 ELISA Kit merges the specificity of sandwich-based ELISA, the sensitivity of real-time PCR, and the easy manipulation of microspheres to detect even the lowest levels of IL-1 beta/IL-1 F2 in serum, plasma, and cell culture supernatant samples.