Lipids

2-(7Z,10Z)-7,10-Hexadecadien-1-yloxirane is an intermediate for the preparation of lipid molecules. It features a polyunsaturated chain with an epoxy ring that can be opened by nucleophiles.

Azido-PEG8-diglycoamide-Lys(Boc)-PAB is a PEG labeling reagent featuring an azide, a Val-Lys dipeptide, and a PAB group. Azides are highly reactive in click chemistry with terminal alkynes and strained cyclooctynes while the Val-Lys dipeptide is enzyme cleavable and triggers payload release via an elimination mechanism within the PAB structure. Finally, the ε-amine of the lysine is Boc-protected.

Azido-PEG8-triethoxysilane is a short PEG linker featuring an azide, which is used in click chemistry with alkynes, and a triethoxysilane group, which may be used to retain a compound on silica surfaces such as those used in normal phase chromatography.

DOPE-PEG-Fluor 555, MW 5,000 is a PEG lipid featuring a DOPE phospholipid and a Fluor 555 dye. DOPE is an unsaturated phospholipid that features prominently in the development of lipid nanoparticles while Fluor 555 is a bright orange cyanine dye that can be used in fluorescence microscopy, FRET and other in vivo imaging techniques.

tert-butyl 3-(7-((undecan-3-yloxy)carbonyl)heptylamino)propylcarbamate is an aminolipid featuring a Boc-protected primary amine, a propylamine spacer attached to an octanoate chain and a C11 chain. This molecule is ionizable at its secondary amine, where it can be alkylated to afford a branched lipid.

N-(Azide-PEG8-carbonyl)-N-bis(PEG8-NHS ester) is a trifunctional reagent that combines an azide and two NHS esters with a tertiary amide junction. The activated NHS ester is reactive towards nucleophiles like amines and alcohols to form amides and esters while the azide is useful for click reactions with terminal alkynes and strained cyclooctynes like DBCO and BCN. The tertiary amide that links the two increases the molecule’s water solubility through hydrogen bonding effects.

BP Lipid 315 is a cationic ionizable lipid ALC-0315 analogue featuring a Boc-protected primary amine, a central tertiary amine, and two ester tails located at the C8 position relative to the amine. One of these esters features a symmetrical branched C17 tail, while the other is an asymmetric C11 tail. Ionizable lipids such as this may be applied in the development of lipid nanoparticles for drug delivery. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

DOPE-PEG-Rhodamine B, MW 2,000 is a PEG-dye-lipid conjugate featuring a DOPE group and a Rhodamine B fluorophore. DOPE is a phospholipid featured prominently in lipid nanoparticles for its ability to readily form micelles while Rhodamine B is a fluorescent dye which is used in histology, FRET and other fluorescence-based assays in vivo.

LNP Trailblazer Kits are designed for quick assembly and assessment of RNA-LNPs in biological systems. Each kit includes a buffer for preparation of RNA cargo and the necessary lipids to form lipid nanoparticles. Once formed RNA-LNPs are stable in neutral pH buffers for several weeks allowing them to be used serially for repeat experiments. The kit components are compatible with bench top microfluidic mixers and hand mixing by pipette.Each LNP Trailblazer kit contains:Your choice of ionizable lipid (selected when ordering)RNA Dilution BufferDSPCCholesterolDMEG-PEG2000The LNP Trailblazer Kit comes with individual lyophilized lipids. General protocols for preparing RNA-LNPs based on a standard eGFP mRNA are included however optimization for each individual RNA or oligonucleotide should be done by the end user. Also available is a kit with pre-mixed lipids in solution (K-6300M)