Lipids

DLin-DMA is an ionizable lipid that is a key lipid component of stable nucleic acid lipid particles (SNALPs). The pKa is 6.0. Reagent grade, for research use only.

4A3-SC8 is an ionizable lipid dendrimer used in generating lipid nanoparticles (LNPs) for RNA delivery. Incorporation of differently charged lipids (DODAP DOTAP or PA) into 4A3-SC8 LNPs allow for selective delivery of RNA to the liver lungs or spleen.

93-O17S is a lipidoid used for generating lipid nanoparticles to deliver RNA peptides and nucleotides. 93-O17S LNPs were able to deliver Cre recombinase mRNA in mice. In addition LNPs were able to deliver the Cre recombinase protein and Cas9:single-guide RNA into cells for genome editing. 93-O17S LNPs were used to deliver the STING pathway agonist 2′3′-Cyclic GMP-AMP (cGAMP) when injected into B16F10 tumors in mice which has potential as an in situ cancer vaccine.

AA3-DLin is an ionizable lipid used in generating lipid nanoparticles (LNPs) for RNA delivery. Unlike LNPs formulated with other ionizable lipids AA3-DLin LNPs performed best with higher proportions of phospholipid and lower cholesterol (40:40:25:0.5 AA3-DLin/DOPE/cholesterol/DMG-PEG). AA3-DLin containing LNPs were able to deliver full-length SARS-COV2 spike protein mRNA to BALB/c mice and generate a strong immune response.

Lipid C24 is an ionizable lipid that can be formulated into lipid nanoparticles for delivery of RNA. Lipid C24 LNPs containing mRNA encoding SARS-CoV-2 Spike protein generated 10X higher binding and pseudoneutralizing antibodies than DLin-MC3-DMA LNPs and showed less injection site inflammation.Reference: US 2022/0218622 A1 "Ionizable lipids and methods of manufacture and use thereof"

YSK05 is an ionizable lipid which is used to make lipid nanoparticles for delivery of siRNA. YSK05 LNPs delivered siRNA to hepatocytes and demonstrated gene silencing after intravenous administration.

DMG-PEG 2000 is a lipid component used in the formulation of lipid nanoparticles (LNPs) for RNA delivery. DMG-PEG 2000 typically makes up a small percentage (0.5-3%) of LNP lipids and its role is to increase the circulatory half-life in vivo.

DSPE-PEG-DBCO, MW 3,400 is a PEG lipid conjugate featuring a DSPE phospholipid and a DBCO group. DSPE is a saturated phospholipid that is commonly used to prepare lipid nanoparticles in drug delivery, while DBCO is a click chemistry handle that is frequently used to conjugate with azides on target molecules to form stable triazole linkages.

Lipid OA2 is an ionizable lipid which can form single component lipid nanoparticles (LNPs) for delivery of RNA. Similar to lipid-based transfection reagents like LipofectamineTM pre-formed OA2 vesicles are incubated with RNA then the mixture is directly added cells. OA2 LNPs delivered siRNA to downregulate cytokine signaling 1 (SOCS1) in mouse bone marrow dentritic cells. These SOCS downregulated DCs were used as a vaccine which reduced tumor size in a B16-OVA mouse model.