Assays & Kits

The ACHE Colorimetric Assay Kit is designed to measure the activity of ACHE (acetylcholinesterase) for screening and profiling applications. The ACHE colorimetric reaction is based on a modified Ellman's method, using the alternative substrate acetylthiocholine iodide (ATCI) and 5,5'-dithio-bis-2-nitrobenzoic acid (DTNB) to quantify amount of thiocholine produced from the hydrolysis of ATCI by ACHE. This assay kit comes in a convenient 96-well format, with purified ACHE enzyme (amino acids 32-614), ATCI, DTNB and assay buffer for 100 enzyme reactions. The absorption intensity of DTNB adduct (λ=410 nm) is used to measure the amount of thiocholine product generated, which is proportional to ACHE activity.

The Chemi-Verse™ ERK1 Kinase Assay Kit is designed to measure ERK1 (extracellular signal-regulated kinase 1) kinase activity for screening and profiling applications using ADP-Glo™ as a detection reagent. The assay kit comes in a convenient 96-well format, with enough purified recombinant ERK1, MBP, ATP, and kinase assay buffer for 100 enzyme reactions.

The Notch1:DLL4 [Biotinylated] Inhibitor Screening Chemiluminescence Assay Kit is designed for screening and profiling molecules that block the binding of Notch1 (neurogenic locus notch homolog protein 1) and DLL4 (delta like canonical notch ligand 4). This kit comes in a convenient 96-well format, with enough recombinant purified Notch1, biotin-labeled DLL4, streptavidin-labeled HRP, and assay buffer for 100 binding reactions. The key to this kit is the high sensitivity of detection of biotin-labeled DLL4 by streptavidin-HRP. Notch1 is coated onto a 96-well plate. After blocking, the plate is pre-incubated with an inhibitor or neutralizing antibody. After incubation with Biotin-DLL4, the plate is washed and Streptavidin-HRP is added. After the ELISA ECL substrate is added the resulting signal can be measured using a chemiluminescence microplate reader. The chemiluminescence signal is proportional to the binding of Notch1 to DLL4.

The CD200:CD200R1 [Biotinylated] Inhibitor Screening Chemiluminescence Assay Kit is designed for screening and profiling molecules that block the binding between CD200 (cluster of differentiation 200) and CD200R1 (CD200 receptor 1). This kit comes in a convenient 96-well format, with enough recombinant human biotin-labeled CD200R1 (amino acids 29-265), human CD200 (amino acids 31-232), streptavidin-HRP, and assay buffer for 100 binding reactions. CD200 is coated onto a 96-well plate. After blocking, the plate is pre-incubated with an inhibitor or neutralizing antibody. After incubation with Biotin-CD200R1, the plate is washed and Streptavidin-HRP is added. After the  ELISA ECL substrate is added  the resulting signal can be measured using a chemiluminescence microplate reader. The chemiluminescence signal is proportional to the binding of CD200 to CD200R1.

The Human NK Cell Isolation Kit is designed to magnetically separate NK cells from a complex immune cell population. This kit is optimized for the isolation of CD56⁺CD3^- cells from normal human peripheral blood mononuclear cells (PBMCs). Cells are incubated with a mix of antibodies followed by conjugation to magnetic beads. They are then placed on a magnet for quick and easy separation. When placed on the magnet, non-NK cells will be immobilized along the side of the tube while NK cells will remain in suspension and can be easily removed for downstream applications.

The PROTAC-Driven Ubiquitination Assay Kit for BET Bromodomains is designed for the testing and profiling of PROTACs targeting the bromodomains on the BET (bromodomain and extraterminal) protein family. After tertiary complex formation between the E3 protein, the PROTAC and the protein of interest, ubiquitination of the target protein is a critical step preceding its degradation. The PROTAC-Driven Ubiquitination Assay Kit for BET Bromodomains comes in an AlphaLISA® format with enough optimized assay buffer, purified recombinant E1 (UBE1), E2 (UbcH5b), and E3 enzymes (Cereblon complex), BRD3 (BD2) (bromodomain-containing protein 3) (amino acids 305-417) as target protein, ATP, and biotinylated ubiquitin for 384 reactions. This kit also contains the PROTAC dBET1 as the control, and JQ1 as control inhibitor. dBET1 or a PROTAC® of interest is incubated with Cereblon complex and BRD3 (BD2), bringing them in close proximity. Ubiquitination reaction occurs following the addition of E2 enzy

The FGR Kinase Assay Kit is designed to measure FGR  kinase activity for screening and profiling applications using ADP-Glo™ as a detection reagent. The assay kit comes in a convenient 96-well format, with enough purified recombinant FGR,  substrate, ATP, and kinase assay buffer for 100 enzyme reactions.

The PARP1 Olaparib Competitive Inhibitor Assay Kit is a competitive FP (fluorescent polarization) assay designed to measure the formation of a complex between PARP1 (poly(ADP-ribose) polymerase 1) and a fluorescent probe that contains the PARP1 inhibitor Olaparib. When the probe is bound to PARP1, FP is high. In the presence of a test compound able to bind to the same site in PARP1 as Olaparib, the Olaparib-containing fluorescent probe is displaced from PARP1 and remains in solution, resulting in low FP. The PARP1 Olaparib Competitive Inhibitor Assay Kit comes in a convenient 96-well format, with purified PARP1 enzyme, Olaparib-containing fluorescent-labeled probe, and assay buffer for 100 enzyme reactions. Note: This kit is not appropriate for inhibitors expected to bind to PARP1 at different sites from Olaparib. PARP1 binds to the Olaparib-containing fluorescent probe, forming a complex. This complex, when subjected to polarized excitation light, emits highly polarized light due to i

The PARP2 Olaparib Competitive Inhibitor Assay Kit is a competitive FP (fluorescent polarization) assay designed to measure the formation of a complex between PARP2 (poly(ADP-ribose) polymerase 2) and a fluorescent probe that contains the PARP2 inhibitor Olaparib. When the probe is bound to PARP1, FP is high. In the presence of a test compound able to bind to the same site in PARP2 as Olaparib, the Olaparib-containing fluorescent probe is displaced from PARP1 and remains in solution, resulting in low FP. The PARP2 Olaparib Competitive Inhibitor Assay Kit comes in a convenient 96-well format, with purified PARP2 enzyme (amino acids 2-583), Olaparib-containing fluorescent-labeled probe, and assay buffer for 100 enzyme reactions. Note: This kit is not appropriate for inhibitors expected to bind to PARP2 at different sites from Olaparib.  PARP2 binds to the Olaparib-containing fluorescent probe, forming a complex. This complex, when subjected to polarized excitation light, emits highly pol